Tau-441 (2N4R) P301S Mutant Monomers (CHO-expressed, N-glycosylated)
Product Sizes
100 ug
SPR-515-100UG
2 x 100 ug
SPR-515-2X100UG
500 ug
SPR-515-500UG
About this Product
- SKU:
- SPR-515
- Additional Names:
- Tau-441, Tau-F, Tau 441, 2N4R, MAPT, TAU, MTBT1, MTBT2, MAPTL, PPND, PPP1R103, FTDP-17, PHF-Tau, Paired Helical Filament-Tau, Neurofibrillary Tangle, NFTs, intracellular neurofibrillary tangles, Tau aggregates, Tau inclusions, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1
- Application:
- Cell-based/Functional Assay, Western Blot
- Buffer:
- 1X PB pH 7.4
- CE/IVD:
- RUO
- Extra Details:
- Tau-441, the longest isoform of the microtubule-associated protein tau (MAPT), consists of 441 amino acids and includes two N-terminal inserts and four microtubule-binding repeat domains. This 2N4R isoform plays a critical role in stabilizing microtubules and maintaining neuronal architecture. The P301S mutation, located within the fourth repeat domain, is a well-characterized pathogenic variant linked to frontotemporal dementia and other tauopathies. Tau-441 P301S mutant monomers exhibit enhanced aggregation propensity and altered conformational dynamics compared to wild-type tau. These properties make them a powerful tool for modeling early-stage tau pathology in neurodegenerative disease research. In vitro and in vivo studies using P301S monomers have demonstrated their ability to initiate misfolding, form toxic oligomers, and seed fibrillar aggregates that mimic neurofibrillary tangles observed in human disease. The P301S mutation accelerates tau self-assembly and disrupts normal microtubule interactions, contributing to synaptic dysfunction, neuronal loss, and neuroinflammation. Tau-441 P301S monomers are widely used in mechanistic studies to investigate tau-mediated toxicity, post-translational modifications, and cellular stress responses. By replicating key molecular features of tau-driven neurodegeneration, Tau-441 P301S mutant monomers support the development of targeted therapies aimed at preventing aggregation, enhancing tau clearance, and restoring neuronal function. Their use advances translational research in Alzheimer's disease, frontotemporal dementia, and related tauopathies. Mammalian N-glycosylation is present on CHO-secreted tau 2N4R, which contributes to slower migration on SDS-PAGE than E.coli or Baculovirus/Sf9 expressed tau (1, 2). N-glycosylated tau has been identified in human AD-diseased brains, but not healthy brains, and may precede tau hyperphosphorylation (3, 4). N-glycosylation of Tau has been demonstrated to affect its aggregation propensity (5). The tau P301S mutation is associated with early onset neurodegeneration, and functionally reduces microtubule assembly and stimulates fibril assembly (6, 7). StressMarq's CHO-expressed Tau 2N4R P301S monomers will readily form fibrils in the absence of heparin and contains mammalian post-translational modifications that may better mimic tau in human AD-brains.
- Immunogen:
- Tau-441 (2N4R) P301S Monomers
- Molecular Weight:
- 48.609 kDa
- Purity:
- >95%
- Purification:
- Affinity Purified and Size Exclusion
- Sequence:
- GGSHHHHHHHHHHGSGGSENLYFQGMAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVSGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -70[o]C
- Supplier:
- StressMarq Biosciences
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
