Tau-441 (2N4R) Wild-Type Monomers (Baculovirus/Sf9)
Product Sizes
100 ug
SPR-496-100UG
2 x 100 ug
SPR-496-2X100UG
5 x 100 ug
SPR-496-5X100UG
About this Product
- SKU:
- SPR-496
- Additional Names:
- MAPT, Tau, Tau-441, 2N4R, Tau40, intracellular neurofibrillary tangles, NFTs, paired helical filaments, PHFs, microtubule-associated Tau, tubulin-associated unit, Paired Helical Filament-Tau, Phf-Tau, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 2
- Application:
- Cell-based/Functional Assay, SDS-PAGE, Western Blot
- Buffer:
- 1X PB pH7.4
- CE/IVD:
- RUO
- Concentration:
- 2 mg/ml
- Extra Details:
- Tau protein, encoded by the MAPT gene (UniProt ID: P10636), is essential for stabilizing microtubules and maintaining neuronal architecture. The Tau-441 isoform, also known as 2N4R, is the longest variant expressed in the adult human brain, containing two N-terminal inserts and four microtubule-binding repeats. This isoform plays a central role in tauopathy research due to its structural complexity and physiological relevance. In its wild-type monomeric form, Tau-441 maintains proper microtubule dynamics and intracellular transport. However, under pathological conditions, even wild-type Tau can undergo conformational changes that lead to misfolding, hyperphosphorylation, and aggregation. These early molecular events are critical to understanding the onset of neurodegenerative diseases such as Alzheimer's disease and frontotemporal dementia. Tau-441 monomers are widely used in experimental models to study the transition from functional protein to pathogenic species. Their behavior under stress, mutation, or post-translational modification provides insight into the mechanisms that drive Tau aggregation and neurotoxicity. These monomers also serve as a baseline for evaluating therapeutic strategies aimed at preserving Tau's native state and preventing disease progression. By modeling the earliest stages of Tau dysfunction, Tau-441 wild-type monomers offer a robust platform for advancing biomarker discovery and the development of targeted interventions in neurodegenerative disease research. Hyper-phosphorylated tau can be generated via expression in the Sf9/Baculovirus system, with up to 20 sites confirmed by mass spectrometry and Western Blots with phospho-specific antibodies(3). Sf9/Baculovirus-expressed Tau 2N4R will readily form oligomers and fibrils in the absence of heparin.
- Immunogen:
- Tau-441 (2N4R) Monomers
- Molecular Weight:
- 45.84 kDa
- Purity:
- >95%
- Purification:
- Ion Exchange
- Sequence:
- MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -70[o]C
- Supplier:
- StressMarq Biosciences
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
- Manufacturer's Data Sheet:https://www.stressmarq.com/products/protein/tau-441-2n4r-wild-type-monomers-baculovirus/sf9-spr-496/
