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Proteins and Peptides

Tau (K18) P301L Mutant Pre-formed Fibrils

Product Sizes
100 ug
SPR-330-100UG
2 x 100 ug
SPR-330-2X100UG
5 x 100 ug
SPR-330-5X100UG
About this Product
SKU:
SPR-330
Additional Names:
Tau K18 P301L, Tau P301L mutant, Tau PFFs, Tau PFF, Tau aggregates, microtubule-associated tau, MAPT, MAP, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 4, tubulin-associated unit, Tau PFFs
Application:
Cell-based/Functional Assay, SDS-PAGE, Western Blot
Buffer:
10 mM HEPES, 100 mM NaCl pH 7.4
CE/IVD:
RUO
Concentration:
2 mg/ml
Extra Details:
Tau is a microtubule-associated protein encoded by the MAPT gene, essential for maintaining neuronal structure and axonal transport. The K18 fragment, comprising the four microtubule-binding repeat domains of tau, serves as a minimal model for studying tau aggregation. The P301L mutation, a proline-to-leucine substitution at residue 301, is linked to familial frontotemporal dementia and significantly enhances tau's aggregation propensity. Pre-formed fibrils (PFFs) generated from Tau (K18) P301L mutant proteins replicate key features of pathological tau aggregates observed in tauopathies. These fibrils act as potent seeds, initiating the misfolding and aggregation of endogenous tau in a prion-like fashion. This seeding process drives the spread of tau pathology across neuronal networks, contributing to synaptic dysfunction, neuroinflammation, and progressive neurodegeneration. In experimental models, Tau (K18) P301L PFFs induce early tau pathology, including the formation of neurofibrillary tangles and tau-positive inclusions. These models are instrumental in elucidating the molecular mechanisms underlying tau propagation, cellular toxicity, and disease progression. The P301L mutation also influences tau's interaction with microtubules and its susceptibility to post-translational modifications, further exacerbating its pathogenic potential. Tau (K18) P301L mutant PFFs are critical tools for preclinical research, enabling high-throughput screening of therapeutic candidates aimed at inhibiting tau aggregation, enhancing clearance, and restoring neuronal function. Their use accelerates the development of targeted interventions for tau-driven neurodegenerative diseases.
Immunogen:
Tau (K18) P301L PFFs
Molecular Weight:
~15.1 kDa
Purity:
>95%
Purification:
Ion Exchange
Sequence:
MSRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE
Shipping Conditions:
Dry Ice
Storage Conditions:
-70[o]C
Supplier:
StressMarq Biosciences
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
TEM of recombinant Tau (K18), P301L mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV=80kV.
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau Pre-formed Fibrils (SPR-330). Tau Pre-formed Fibrils (SPR-330) seed the formation of new tau fibrils when combined with tau monomers (SPR-327). Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.
SDS-PAGE of ~15 kDa Active Human Tau Protein K18 P301L Pre-formed Fibrils (SPR-330). Lane 1: MW Ladder. Lane 2: Tau Protein Pre-formed Fibrils (SPR-330).