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Proteins and Peptides

Tau-441 (2N4R) P301S Mutant Monomers

Product Sizes
100 ug
SPR-327-100UG
2 x 100 ug
SPR-327-2X100UG
5 x 100 ug
SPR-327-5X100UG
About this Product
SKU:
SPR-327
Additional Names:
Tau-441, Tau-F, Tau 441, 2N4R, MAPT, TAU, MTBT1, MTBT2, MAPTL, PPND, PPP1R103, FTDP-17, PHF-Tau, Paired Helical Filament-Tau, Neurofibrillary Tangle, NFTs, intracellular neurofibrillary tangles, Tau aggregates, Tau inclusions, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1
Application:
Cell-based/Functional Assay, SDS-PAGE, Western Blot
Buffer:
10 mM HEPES, 100 mM NaCl pH 7.4
CE/IVD:
RUO
Concentration:
2 mg/ml
Extra Details:
Tau-441, also known as the 2N4R isoform of the microtubule-associated protein tau (MAPT), is the longest isoform expressed in the adult human brain. It plays a critical role in stabilizing microtubules and maintaining neuronal structure and function. Under physiological conditions, tau monomers dynamically bind microtubules, supporting axonal transport and synaptic integrity. The P301S mutation, a proline-to-serine substitution at residue 301, is associated with familial frontotemporal dementia and other tauopathies. This mutation alters the conformation and biochemical properties of tau monomers, reducing their affinity for microtubules and increasing their susceptibility to misfolding and aggregation. P301S mutant monomers exhibit enhanced nucleation potential, facilitating the formation of toxic oligomers and fibrils that disrupt neuronal homeostasis. These misfolded tau species interfere with cytoskeletal dynamics, impair axonal transport, and activate neuroinflammatory pathways, contributing to progressive neurodegeneration. In transgenic models, P301S tau monomers initiate early pathological changes, including synaptic loss, mitochondrial dysfunction, and cognitive decline, even before the appearance of mature neurofibrillary tangles. Understanding the molecular behavior of P301S mutant tau monomers is essential for developing targeted therapies. Strategies aimed at stabilizing tau's native conformation, inhibiting early aggregation events, or enhancing clearance mechanisms are under investigation to mitigate tau-driven neurodegeneration. Tau-441 P301S mutant monomers represent a critical early target in the cascade of tauopathy progression, offering valuable insights for therapeutic intervention and biomarker development.
Immunogen:
Tau-441 (2N4R) P301S Monomers
Molecular Weight:
~45.8 kDa
Purity:
>95%
Purification:
Ion Exchange
Sequence:
MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TPPKSPSSAK SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVSGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMV DSPQLATLAD EVSASLAKQG L
Shipping Conditions:
Dry Ice
Storage Conditions:
-70[o]C
Supplier:
StressMarq Biosciences
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Monomer (SPR-327). Lane 1: MW ladder. Lane 2: Tau Protein Monomer (SPR-327)
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau monomers (SPR-327). A greater increase in fluorescence is seen when 50 uM monomer (SPR-327) is combined with 10 nM PFFs (SPR-329 or SPR-330), as the fibrils seed the formation of new fibrils from the pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.