Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Proteins and Peptides

Alpha Synuclein Monomers (Type 1)

Product Sizes
100 ug
SPR-323-100UG
2 x 100 ug
SPR-323-2X100UG
500 ug
SPR-323-500UG
About this Product
SKU:
SPR-323
Additional Names:
Alpha-synuclein, Alpha synuclein, Asyn, SNCA, NACP, PARK1, PARK4, PD1, Synuclein alpha, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Synuclein Alpha-140, SYN, Parkinson's disease familial 1 Protein Protein
Application:
Cell-based/Functional Assay, SDS-PAGE, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Extra Details:
Alpha-synuclein (A Alpha-syn), a 140-amino acid neuronal protein encoded by the SNCA gene, plays a vital role in maintaining synaptic function. In its monomeric form, A Alpha-syn regulates synaptic vesicle trafficking, neurotransmitter release, and SNARE-complex assembly, contributing to efficient neuronal communication and dopamine homeostasis. Under physiological conditions, A Alpha-syn monomers exhibit a dynamic, unfolded structure that allows interaction with lipid membranes and synaptic proteins. However, environmental stressors, genetic mutations, and aging can destabilize this native conformation, triggering misfolding and self-aggregation. These misfolded monomers act as seeds for the formation of toxic oligomers and fibrils, initiating a prion-like propagation of pathology across neural networks. This pathological transformation disrupts synaptic integrity, impairs mitochondrial function, and activates neuroinflammatory pathways, ultimately leading to neuronal death. Monomeric A Alpha-syn is increasingly recognized as a critical upstream factor in the development of synucleinopathies, including Parkinson's disease, Lewy body dementia, and multiple system atrophy. Targeting A Alpha-syn monomers before aggregation occurs offers a promising therapeutic strategy. Approaches such as molecular chaperones, SNCA gene modulation, and immunotherapies aim to stabilize monomeric A Alpha-syn or prevent its pathological conversion. As research advances, understanding the molecular behavior of A Alpha-syn monomers is essential for developing disease-modifying treatments and biomarkers for early diagnosis in neurodegenerative disorders.
Immunogen:
Alpha Synuclein Monomers
Molecular Weight:
~14.46 kDa
Purity:
>95%
Purification:
Ion Exchange
Sequence:
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP GSEAYEMPSE EGYQDYEPEA
Shipping Conditions:
Dry Ice
Storage Conditions:
-70[o]C
Supplier:
StressMarq Biosciences
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers (SPR-323). (B) 2.5 uL mouse alpha synuclein monomers (SPR-323). (C) 2.5 ug alpha synuclein PFFs (SPR-324). (C) 5 ug alpha synuclein PFFs (SPR-324) Inset: PBS (negative control). Primary antibody: Anti-Alpha Synuclein pSer129 (SMC-600) at 1:10 000. Secondary antibody: anti-rabbit HRP. Mice injected with PFF displayed alpha synuclein staining in the striatum and cortex and contralateral to the injection site. Courtesy of: Porsolt.
Type 1 alpha synuclein pre-formed fibrils (SPR-324) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (SPR-323). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 uM of Type 1 alpha synuclein pre-formed fibrils (SPR-324) is combined with 100 uM of alpha synuclein monomer (SPR-323), as compared to Type 1 alpha synuclein pre-formed fibrils (SPR-324) or alpha synuclein monomer (SPR-323) alone. Thioflavin T ex = 450 nm, em = 485 nm.
SDS-PAGE of ~14 kDa Mouse Recombinant Alpha Synuclein Protein Monomer (SPR-323). Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Monomer (2 ug) (SPR-323).