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Proteins and Peptides

Alpha Synuclein Monomers (Type 1)

Product Sizes
100 ug
SPR-321-100UG
2 x 100 ug
SPR-321-2X100UG
500 ug
SPR-321-500UG
About this Product
SKU:
SPR-321
Additional Names:
Alpha-synuclein, Alpha synuclein, Asyn, SNCA, NACP, PARK1, PARK4, PD1, Synuclein alpha, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Synuclein Alpha-140, SYN, Parkinson's disease familial 1 Protein Protein
Application:
Cell-based/Functional Assay, SDS-PAGE, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Extra Details:
Alpha-synuclein (A Alpha-syn), a 140-amino acid neuronal protein encoded by the SNCA gene, plays a pivotal role in synaptic vesicle trafficking, neurotransmitter release, and SNARE-complex assembly. In its native monomeric form, A Alpha-syn enhances synaptic vesicle priming and fusion, contributing to efficient neuronal communication. However, dysregulation of A Alpha-syn monomers is increasingly recognized as a critical early event in the pathogenesis of neurodegenerative disorders, particularly synucleinopathies such as Parkinson's disease (PD), Lewy body dementia (LBD), and multiple system atrophy (MSA). [www.uniprot.org] Recent studies reveal that monomeric A Alpha-syn can misfold and aggregate into toxic oligomers and fibrils, initiating a prion-like cascade of intracellular and intercellular propagation. This aggregation disrupts synaptic integrity, impairs dopamine neurotransmission, and triggers neuroinflammation, ultimately leading to neuronal death. Moreover, mutations in SNCA and environmental factors such as exposure to neurotoxins (e.g., rotenone) exacerbate A Alpha-syn pathology, accelerating disease progression. [www.mdpi.com], [link.springer.com] [www.frontiersin.org] Understanding the molecular dynamics of A Alpha-syn monomers, including their interactions with membrane receptors like P2RX7, offers promising avenues for therapeutic intervention. Targeting monomeric A Alpha-syn before pathological aggregation occurs may provide a strategic window for disease-modifying therapies. As research advances, A Alpha-syn monomers continue to emerge as a focal point in unraveling the mechanisms of neurodegeneration and developing precision treatments for synucleinopathies.
Immunogen:
Alpha Synuclein Monomers
Molecular Weight:
~14.46 kDa
Purity:
>95%
Purification:
Ion Exchange
Sequence:
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Shipping Conditions:
Dry Ice
Storage Conditions:
-70[o]C
Supplier:
StressMarq Biosciences
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
Type 1 alpha synuclein pre-formed fibrils (SPR-322) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (SPR-321). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of Type 1 alpha synuclein pre-formed fibrils (SPR-322) is combined with 100 uM of alpha synuclein monomer (SPR-321), as compared to when 10 nM of Type 1 alpha synuclein pre-formed fibrils (SPR-322) is combined with 100 uM of alpha Synuclein monomer (SPR-316). Thioflavin T ex = 450 nm, em = 485 nm.
SDS-PAGE of ~14 kDa Human Recombinant Alpha Synuclein Protein Monomer (SPR-321). Lane 1: Molecular Weight Ladder (MW). Lane 2: Alpha Synuclein Protein Monomer (2 ug) (SPR-321).