DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG[R] M2 Antibody): HRP
Product Sizes
100 ug
SPC-720D-HRP-100UG
About this Product
- SKU:
- SPC-720D-HRP
- Additional Names:
- DDDDK epitope tag, DDDK, ddk, DYKDDDDK, DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG, FLAG tag
- Application:
- Western Blot
- Buffer:
- 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
- CE/IVD:
- RUO
- Clonality:
- Polyclonal
- Conjugate:
- HRP
- Concentration:
- 1 mg/ml
- Extra Details:
- The DYKDDDDK tag, also known as the FLAG® tag, is a widely used epitope tag in molecular biology and protein research. Comprising eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), this hydrophilic sequence is engineered into recombinant proteins to facilitate detection, purification, and localization studies. Its small size minimizes interference with protein function, making it ideal for fusion protein applications in both prokaryotic and eukaryotic systems. Anti-DYKDDDDK tag antibodies, such as those equivalent to Sigma's Anti-FLAG® M2, are monoclonal or polyclonal antibodies specifically designed to recognize the DYKDDDDK epitope with high affinity and specificity. These antibodies are essential tools in Western blotting, immunoprecipitation, immunofluorescence, and ELISA, enabling researchers to track tagged proteins with precision. The FLAG tag system is particularly valued for its compatibility with gentle elution conditions, preserving protein structure and function during purification. Additionally, the tag's unique sequence ensures minimal cross-reactivity with endogenous proteins, enhancing signal clarity in complex biological samples. Anti-DYKDDDDK antibodies are validated across multiple species and applications, making them a cornerstone in proteomics, structural biology, and therapeutic protein development. Their robust performance and commercial availability have established them as a gold standard in epitope tagging technologies.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH).
- Purification:
- Affinity Purified
- Reactivities:
- Wide/Non-species Specific
- Shipping Conditions:
- Blue Ice
- Specificity:
- Binds to DYKDDDDK (FLAG tag) conjugated proteins. Recongizes the same epitope as Sigma's Anti-FLAG® antibodies.
- Storage Conditions:
- See Manual
- Supplier:
- StressMarq Biosciences
- Type:
- Antibody: Polyclonal Antibody
