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Antibodies

Calcium Sensing Receptor Antibody: ATTO 390

Product Sizes
100 ug
SPC-713D-A390-100UG
About this Product
SKU:
SPC-713D-A390
Additional Names:
Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism Antibody, HHC Antibody, Extracellular calcium sensing receptor Antibody, FIH Antibody, Parathyroid Cell calcium-sensing receptor Antibody, CASR Antibody, CASR_HUMAN Antibody, Ca sensing receptor Antibody, Extracellular calcium-sensing receptor Antibody, PCAR1 Antibody, FHH Antibody, Ca2+ sensing receptor Antibody, EIG8 Antibody, Hypocalciuric hypercalcemia 1 Antibody, PCaR1 Antibody, GPRC2A Antibody, Extracellular calcium sensing receptor [Precursor] Antibody, PCAR 1 Antibody, Parathyroid cell calcium-sensing receptor 1 Antibody, HHC1 Antibody, Parathyroid Cell calcium sensing receptor Antibody, CAR Antibody, MGC138441 Antibody, NSHPT Antibody, CaSR Antibody, Ca2+ sensing receptor 1 Antibody, Parathyroid Ca(2+) sensing receptor 1 Antibody
Application:
Immunocytochemistry, Immunofluorescence, Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
ATTO 390
Concentration:
1 mg/ml
Host:
Rabbit
Immunogen:
Synthetic peptide from the N-terminal (1-100 aa) of Human calcium-sensing receptor (CASR)
Purification:
Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Specificity:
Detects ~140 kDa.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Polyclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Calcium Sensing Receptor Antibody (D) Composite.
Western blot analysis of Human HeLa cell lysates showing detection of 130kDa Calcium Sensing Receptor protein using Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 130kDa.