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Antibodies

ATG9B Antibody

Product Sizes
12 ug
SPC-646-12UG
100 ug
SPC-646-100UG
About this Product
SKU:
SPC-646
Additional Names:
APG9-like 2 Antibody, APG9L2 Antibody, Nitric oxide synthase 3-overlapping antisense gene protein Antibody, Protein sONE Antibody, NOS3AS Antibody, ATG9B Antibody, Autophagy-related protein 9B Antibody
Application:
Immunocytochemistry, Immunofluorescence, Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
ATG9B is involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Highly expressed in the placenta and the pituitary gland.
Host:
Rabbit
Immunogen:
Synthetic peptide from the N-terminal of Human ATG9B (aa. 110-121)
Purification:
Affinity Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Specificity:
Detects ~100 kDa.
Storage Conditions:
-20[o]C
Supplier:
StressMarq Biosciences
Type:
Antibody: Polyclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG9B Antibody (D) Composite.
Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.