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Antibodies

Amyloid Beta 1-42 Oligomer Antibody, Clone 1A2

Product Sizes
12 ug
SMC-618-12UG
100 ug
SMC-618-100UG
About this Product
SKU:
SMC-618
Additional Names:
Amyloid Beta 1-42 Oligomer, AB Beta1-42 Oligomer, Abeta Oligomers, Amyloid beta peptide 1-42, AB Beta42, Beta-amyloid protein 42, Beta-APP42, Amyloid beta A4 protein, APP, ABPP, APPI, Non-A4 component of amyloid precursor, Alzheimer disease amyloid protein
Application:
ELISA, Western Blot
Buffer:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
CE/IVD:
RUO
translate.label.attr.clone:
1A2
Clonality:
Recombinant Monoclonal
Concentration:
1 mg/ml
Extra Details:
Amyloid beta (AB Beta) peptide, particularly the 1-42 isoform (AB Beta1-42), plays a central role in the pathogenesis of Alzheimer's disease (AD). AB Beta is generated through proteolytic cleavage of the amyloid precursor protein (APP) and can self-assemble into a range of aggregated species, including oligomers, protofibrils, fibrils, and ultimately extracellular plaques. Among these, AB Beta1-42 oligomers are increasingly recognized as the most neurotoxic species, contributing to synaptic dysfunction, neuroinflammation, and cognitive decline. Our amyloid beta oligomer antibody has been validated for high specificity toward AB Beta1-42 oligomers, distinguishing them from other aggregated forms. This specificity is critical for accurately detecting early-stage, soluble aggregates that precede plaque formation and are believed to drive early neurodegenerative changes in AD (1). The immunogen used to generate these oligomers was Amyloid Beta Peptide 1-42, pre-treated with 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP), following established protocols to ensure reproducible oligomer formation (2, 3). This preparation method enhances the structural fidelity of oligomeric species, enabling more precise investigation of their pathological roles. Given that most therapeutic strategies over the past two decades have targeted AB Beta accumulation, tools that selectively recognize AB Beta1-42 oligomers are essential for advancing both basic research and translational efforts in Alzheimer's disease.
Host:
Rabbit
Immunogen:
Amyloid beta 1-42 oligomers (StressMarq Catalog# SPR-488)
Isotype:
IgG
Purification:
Protein A Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Specificity:
Amyloid Beta 1-42 oligomers
Storage Conditions:
-20[o]C
Supplier:
StressMarq Biosciences
Type:
Antibody: Recombinant Antibodies
Immunohistochemistry (IHC) analysis on a temporal lobe section of a human Alzheimer's diseased brain tissue. Block: 3% skim milk in PBST. Primary Antibody: StressMarq's Rabbit Anti-Amyloid Beta Oligomer Monoclonal Antibody, clone 1A2 (SMC-618) at 1:100. Secondary: Goat-anti-rabbit IgG:HRP at 1:100. Stained with DAB (1:50) and Hematoxylin (1:10).
Western blot analysis comparing detection of StressMarq's Amyloid Beta 1-42 constructs (SPR-485 = amyloid beta 1-42 peptide; SPR-488 = amyloid beta 1-42 oligomers; SPR-487 = amyloid beta 1-42 pre-formed fibrils; SPR-492 = amyloid beta pyroglutamate 3-42 pre-formed fibrils). Block: 5% skim milk. Primary Antibodies: Rabbit Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (SMC-618, clone 1A2) & Mouse Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10), both at 1:1000 for 1/2 hour at RT. Secondary Antibodies: Goat anti-rabbit IgG:HRP or anti-mouse IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.
Sandwich ELISA analysis comparing detection of Amyloid Beta constructs. The range tested for each construct was 0 to 2000 pg/mL, and the standard curve was prepared using amyloid beta 1-42 oligomers (SPR-488). Within this range, no cross-reactivity with monomeric peptide & APP 751 was observed.
Dot blot analysis comparing detection of StressMarq's amyloid beta 1-42 constructs, alpha synuclein wildtype constructs, and tau constructs. Of note is that SMC-618 does not bind APP 751 or other protein constructs such as alpha synuclein and tau. Block: 5% skim milk. Primary Antibodies: Anti-Amyloid Beta 1-42 oligomer monoclonal antibody (SMC-618, clone 1A2), Anti-Amyloid Beta 1-16 monoclonal antibody (Biolegend 803001, clone 6E10), Anti-Alpha Synuclein polyclonal antibody (SPC-800), and Anti-Tau polyclonal antibody (SPC-801), all incubated for 1/2 hour at RT. Secondary Antibodies: Goat anti-rabbit IgG:HRP or anti-mouse IgG:HRP at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min in RT. Exposed 1 second.