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Antibodies

Alpha Synuclein Antibody (Aggregate-Specific), Clone 2F11

Product Sizes
12 ug
SMC-617-12UG
100 ug
SMC-617-100UG
About this Product
SKU:
SMC-617
Additional Names:
Alpha Synuclein Antibody, Non-A beta component of AD amyloid Antibody, Non-A4 component of amyloid precursor Antibody, NACP Antibody, SNCA Antibody, PARK1 Antibody, PARK 1 Antibody, alphaSYN Antibody, PARK 4 antibody, PARK4 antibody, Parkinson disease familial 1 antibody, Parkinson disease (autosomal dominant, Lewy body) 4 antibody, SYN antibody
Application:
Conjugation/Labeling, Dot Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS pH7.4, 50% glycerol, 0.09% sodium azide
CE/IVD:
RUO
translate.label.attr.clone:
2F11
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer's disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). Covered by US patent family US11,098,108B2 (7).
Host:
Mouse
Immunogen:
Mouse alpha synuclein fibrils
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Human, Mouse
Shipping Conditions:
Blue Ice
Specificity:
This antibody binds a 3-dimensional fibrillar epitope of alpha synuclein (including AA101-120, AA61-80), present in MSA (Multiple System Atrophy) aggregates in human brain samples. It does not appear to detect PD (Parkinson's disease) or PDLB (Parkinson's disease with Lewy Bodies) aggregate structures in human brain samples. It can, however, bind recombinant human PFFs and mouse PFFs.
Storage Conditions:
-20[o]C
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immuno-gold electron micrograph of sonicated A Alpha-Syn Fibrils (SPR-322) stained with 2F11 antibody (1:400 dilution) and visualized using goat anti-mouse IgG conjugated with 18 nm gold particles. The sample was then placed on a transmission electron microscopy metallic grid and stained with uranyl acetate.
Western blot on 15ug mouse brain lysate from Fmr1 KO mice (FXS mice) with C57Bl/6J genetic background. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody clone 2F11 at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Band clearly identifies large molecular weight oligomeric band (monomeric band predicted at 14kD).
Western blot on 15ug Hela lysate. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody clone 2F11 at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Band clearly identifies large molecular weight oligomeric band (monomeric band predicted at 14kD).
SPR binding analysis of 2F11 antibody and human alpha synuclein type 1 fibrils (SPR-322), type 2 (SPR-317) fibrils and monomeric protein (SPR-321). Buffer (top left), alpha synuclein monomers (top right), alpha synuclein fibrils type1 (bottom left), and alpha synuclein fibrils type 2 (bottom right). Data: KD[nM] for SPR-322 = 42.5, SPR-317 = 365.3; 2F11 binds SPR-322 with 8.6 x greater affinity than SPR-317. 2F11 does not bind monomeric alpha synuclein (SPR-321).
8 Day differentiated iPSCs with added alpha synuclein fibrils (SPR-322), conjugated to Alexa Fluor 555 with or without 1h prior 2F11 addition. SPR-322 Alexa Fluor 555 added at 5ug/well for 48h, 2F11 (50ug/well added 1h before fibrils where applicable). Cells were subsequently washed 48h after addition and imaged a following 24h later. Imaging and channels: 2F11 antibody: 647nm (using an anti-mouse-647 conjugate); Fibrils: 555nm, phosph-ser129 (SPR-600) 488nm, and Hoechst stain for DNA. Image 4a top, no fibrils, no 2F11 added. Bottom, fibrils added, no 2F11 antibody. Fibrils are clearly visible in the cells as is enhanced phosph-ser129 signal. Image 4b top, no fibrils, 2F11 antibody added. Bottom, fibrils and 2F11 antibody added. The image clearly shows 2F11 antibody addition stops cell entry of the 555-labelled fibrils.