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Antibodies

DENND4C Antibody: RPE

Product Sizes
100 ug
SMC-610D-RPE-100UG
About this Product
SKU:
SMC-610D-RPE
Additional Names:
DENN domain-containing protein 4C antibody
Application:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
3E8
Clonality:
Monoclonal
Conjugate:
R-PE
Concentration:
1 mg/ml
Extra Details:
Dennd4c is a guanine nucleotide exchange factor (GEF) for Rab10, and required for GLUT4 translocation (1). It is associated with Alkuraya-Kucinskas Syndrome and Koolen-De Vries Syndrome (2) and interacts with the retromer (3).
Host:
Mouse
Immunogen:
Full Length Human Recombinant Dennd4c protein
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~213 kDa.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 (SMC-610). Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody (SMC-610) at 1:100, 1:500, 1:1000 for 1 hour at RT. Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:1000 for 1 hour at RT. Counterstain: DAPI. Courtesy of: Dario Alessi Lab, University of Dundee.
Immunoprecipitation analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 (SMC-610). Tissue: A549, MEF cells. Species: Human, Mouse. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody (SMC-610). Three amounts of SMC-610 (10, 3, and 1 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 or MEF lysate for 2 hours at 4°C. Courtesy of: Dario Alessi Lab, University of Dundee.
Western Blot analysis of Human A549, MEF cells showing detection of DENND4C protein using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 (SMC-610). Lane 1: MW ladder. Lane 2: A549 WT. Lane 3: A549 DENND4C KO. Lane 4: MEF. Load: 30 ug. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody (SMC-610) at 1:1000. Courtesy of: Dario Alessi Lab, University of Dundee.