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Antibodies

7-Ketocholesterol Antibody, Clone 7E1: PerCP

Product Sizes
100 ug
SMC-512D-PCP-100UG
About this Product
SKU:
SMC-512D-PCP
Additional Names:
7-Ketocholesterol [7-KC] Antibody, 7-KC Antibody, 7-ketocholesterol (7-KC) Antibody, 7KC Antibody, 7KC-Modified Keyhole-Lympet Hemocyanine Antibody, 7KC-Modified KLH Antibody
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
95.46mM Phosphate, 2.48mM MES and 2mM EDTA
CE/IVD:
RUO
translate.label.attr.clone:
7E1
Clonality:
Monoclonal
Conjugate:
PerCP
Concentration:
1 mg/ml
Extra Details:
Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 7-ketocholesterol (7-KC) is a biomarker of lipoprotein cholesterol oxidation.
Host:
Mouse
Immunogen:
Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG2b
Purification:
Protein G Purified
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Specificity:
Specific for 7-ketocholesterol modified proteins.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 7E1 (SMC-512). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody (SMC-512) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol protein using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 7E1 (SMC-512). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (2.0 ug). Lane 4: 7-ketocholesterol-BSA (0.5 ug). Lane 5: 7-ketocholesterol-BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody (SMC-512) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.