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Antibodies

4-Hydroxy-2-hexenal Antibody, Clone 6F10: RPE

Product Sizes
100 ug
SMC-510D-RPE-100UG
About this Product
SKU:
SMC-510D-RPE
Additional Names:
4-Hydroxy-2-hexenal, 4-Hydroxyhex-2-enal, 4-Hydroxyhexenal, 4-Hydroxy Hexenal, Hex-2-enal, 4-hydroxy-, Hexenal, 4-hydroxy-, 2-Hexenal, 4-hydroxy-, (E)-, (2E)-4-Hydroxy-2-hexenal, (E)-4-Hydroxy-2-hexenal
Application:
ELISA, FACS, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
95.46mM Phosphate, 2.48mM MES and 2mM EDTA
CE/IVD:
RUO
translate.label.attr.clone:
6F10
Clonality:
Monoclonal
Conjugate:
R-PE
Concentration:
1 mg/ml
Extra Details:
Lipid peroxidation, driven by oxidative stress, targets carbon-carbon double bonds in polyunsaturated fatty acids (PUFAs), leading to membrane damage and the generation of reactive aldehydes. Among these, 4-Hydroxy-2-hexenal (4-HHE) is a major A Alpha,B Beta-unsaturated aldehyde derived specifically from the oxidation of n-3 PUFAs. Emerging evidence highlights 4-HHE as a critical mediator of neuronal injury and a potential biomarker in neurodegenerative disease. 4-HHE is highly electrophilic and forms stable Michael addition adducts with nucleophilic amino acid residues, particularly histidine. These covalent modifications disrupt protein structure and function, impairing key cellular processes such as mitochondrial respiration, synaptic signaling, and proteostasis. Elevated levels of 4-HHE have been observed in various pathological states, including Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis (ALS), implicating it in the progression of oxidative damage and chronic neuroinflammation. In neuroscience research, 4-HHE is gaining attention for its dual role as both a marker of lipid peroxidation and an active contributor to neurotoxicity. Its ability to alter neuronal viability and promote apoptotic pathways underscores its relevance in the study of aging and neurodegeneration. Understanding the molecular mechanisms of 4-HHE toxicity is essential for developing targeted antioxidant therapies and early diagnostic tools in neurodegenerative disease research.
Host:
Mouse
Immunogen:
Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG2b
Purification:
Protein G Purified
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Specificity:
Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10 (SMC-510). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody (SMC-510) at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Western Blot analysis of 4-hydroxy-2-hexanal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-2-hexenal protein using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10 (SMC-510). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: 4-hydroxyl nonenal-BSA (0.5 ug). Lane 4: 4-hydroxy nonenal-BSA (2.0 ug). Lane 5: 4-hydroxy-2-hexenal (0.5 ug). Lane 6: 4-hydroxy-2-hexenal (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody (SMC-510) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Flow Cytometry analysis using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10 (SMC-510). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody (SMC-510) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 uM H2O2 for 24 hours.