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Antibodies

GABA A Receptor Alpha 5 Antibody, Clone N415/24: PerCP

Product Sizes
100 ug
SMC-494D-PCP-100UG
About this Product
SKU:
SMC-494D-PCP
Additional Names:
Gamma-aminobutyric acid receptor subunit alpha-5 Antibody, GABA(A) Receptor subunit Alpha-5 Antibody, Gabra5 Antibody
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
N415/24 (Formerly sold as S415-24)
Clonality:
Monoclonal
Conjugate:
PerCP
Concentration:
1 mg/ml
Extra Details:
The GABA A Receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). Recent research shows their usage in investigating side effects of drugs and the effects of learning and memory. Inverse agonists of the Alpha5 subunit may be useful in the treatment of Alzheimer's Disease (3).
Host:
Mouse
Immunogen:
Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone N415/24 (SMC-494). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody (SMC-494) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA A Receptor Alpha 5 Antibody (D) Composite.
Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone N415/24 (SMC-494). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody (SMC-494) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.