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Antibodies

GABA-A Receptor Alpha 2 Antibody, Clone N399/19: ATTO 390

Product Sizes
100 ug
SMC-486D-A390-100UG
About this Product
SKU:
SMC-486D-A390
Additional Names:
GABA A receptor subunit alpha 2 Antibody, GABRA2 Antibody, Gamma aminobutyric acid A receptor Alpha 2 Antibody, GBRA2_Human Antibody
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
N399/19 (Formerly sold as S399-19)
Clonality:
Monoclonal
Conjugate:
ATTO 390
Concentration:
1 mg/ml
Extra Details:
The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).
Host:
Mouse
Immunogen:
Fusion protein amino acids 350-385 (Cytoplasmic C-terminus) of rat GABA-A Receptor Alpha2
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~55kDa. Does not cross-react with GABA-A Receptor Alpha1.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 2 Monoclonal Antibody, Clone N399/19 (SMC-486). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 2 Monoclonal Antibody (SMC-486) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 2 Antibody (D) Composite.
Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 2 protein using Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody, Clone N399/19 (SMC-486). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody (SMC-486) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa. Other Band(s): ~ 37 kDa, ~50 kDa, ~70 kDa.
Western Blot analysis of Rat Brain showing detection of ~55 kDa GABA A Receptor Alpha 2 protein using Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody, Clone N399/19 (SMC-486). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 ug. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody (SMC-486) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~55 kDa.