Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

GABA A Receptor Beta 3 Antibody, Clone N87/25: RPE

Product Sizes
100 ug
SMC-339D-RPE-100UG
About this Product
SKU:
SMC-339D-RPE
Additional Names:
ECA5 antibody, GABA alpha receptor beta-2 subunit antibody, GABA(A) receptor subunit beta-3 antibody, GABAA receptor beta 3 subunit antibody, GABAA receptor subunit beta 3 antibody, GABR B3 antibody, Gabrb3 antibody, Gamma aminobutyric acid (GABA) A receptor beta 3 antibody, Gamma aminobutyric acid receptor subunit beta 3 antibody, Gamma-aminobutyric acid receptor subunit beta-3 antibody, GBRB3_HUMAN antibody, MGC9051 antibody
Application:
Antibody Microarray, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
N87/25 (Formerly sold as S87-25)
Clonality:
Monoclonal
Conjugate:
R-PE
Concentration:
1 mg/ml
Extra Details:
The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).
Host:
Mouse
Immunogen:
Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta 3
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~55kDa. No cross-reactivity against GABA-A-R-Beta 2 or -Beta1.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody, Clone N87/25 (SMC-339). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Beta 3 Monoclonal Antibody (SMC-339) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Beta 3 Antibody (D) Composite.
Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25 (SMC-339). Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-339) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
Western Blot analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N87/25 (SMC-339). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-339) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.