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Antibodies

F(ab')2 Rabbit IgG (H&L) Antibody Pre-Adsorbed

Product Sizes
500 ug
711-1122-500UG
About this Product
SKU:
711-1122
Additional Names:
Goat F(ab')2 Anti-Rabbit IgG Antibody Pre-Adsorbed, Goat Fab2 Anti-Rabbit IgG Antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Rabbit IgG whole molecule
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Rabbit
Shipping Conditions:
Blue Ice
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
SDS-PAGE results of Goat F(ab')2 Anti-Rabbit IgG (H&L) Antibody. Lane 1: reduced F(ab')2 anti-Mouse (lot# 36997). Lane 2: reduced F(ab')2 anti-Rabbit (lot# 36998). Lane 3: Opal Prestained Ladder - MB-210-0500. Lane 4: non-reduced F(ab')2 anti-Mouse. Lane 5: non-reduced F(ab')2 anti-Rabbit. Load: 1.0ug. 4-20% Lonza SDS-PAGE; Coomassie Stained; BioRad ChemiDoc Imaged.
Western Blot results using Goat F(ab')2 Anti-Rabbit IgG. Effects of RAB38 silencing on the proliferation ability of PANC-1 and BxPC-3 cells. The expression levels of the proliferation-associated proteins (C) Ki67 and (D) PCNA in PANC-1 and BxPC-3 cells were detected by western blot analysis. Data are reported as the mean +/- standard deviation (n=3). *P<0.05 vs. control group. shRNA, small hairpin RNA; RAB38, Ras-related GTP-binding protein 38; OD, optical density; PCNA, proliferating cell nuclear antigen. Figure 3. PMID: 30569114.
KIF20B shRNA efficiency. (B) The shRNA efficiency measured by western blotting. Primary antibodies used rabbit anti-KIF20B antibody [1:100] and mouse anti-B Beta-actin [1:500] with goat anti-rabbit [1:10,000] p/n 711-1122 and goat anti-mouse Fc secondary antibody [1:10,000] p/n 110-1103. *P<0.05 vs. control. KIF, kinesin family member; shRNA, short hairpin RNA. Figure 2. PMID: 30664160.