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Antibodies

Cyclin D3 Antibody

Product Sizes
25 ul
600-401-GN2S-25UL
About this Product
SKU:
600-401-GN2S
Additional Names:
Ccnd3|rabbit anti-Cyclin D3 antibody, G1/S-specific cyclin-D3, Ccnd3, Cyl-3
Application:
ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Anti-Cyclin D3 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Cyclin D3 belongs to a highly conserved cyclin family, whose members are the ultimate recipients of oncogenic signals. Cyclin D3 is a key component of the cell cycle progression machinery and induces progression through the G1 phase of the cell cycle. Cyclin D3 is expressed in nearly all proliferating cells, and shows the most broad expression pattern of all three D-type (D1-D3) cyclins. Cyclin D3 is encoded from the 6p21 chromosome region and the protein is predominantly localized in the nucleus. Once induced, cyclin D3 binds and activates its associated cyclin-dependent kinases CDK4 and CDK6. Amplification of the cyclin D3 gene and overexpression of cyclin D3 protein is seen in several human cancers. A large number of human malignancies contain lesions in pathways impacting on cyclin D3. Abnormal expression of Cyclin D3 is believed to be a driving force in several human cancers. A possible role for cyclin D3 in the malignancies of the lymphoid system is suggested by the observations that cyclin D3 gene is rearranged in several neoplastic diseases such as diffuse large B cell lymphomas or multiple myelomas. Anti-Cyclin D3 is ideal for researchers interested in Cancer Research and Immunology research.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Cyclin D3 protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Cyclin D3 affinity-purified antibody is directed against Cyclin D3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated Cyclin D3.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry with anti-Cyclin D3 antibody showing Cyclin D3 staining in nucleus of lymphocytes in human spleen at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-mouse Cyclin D3 antibody at 4.0 ug/ml for 60 minutes. The reaction was developed using MACH 4 universal AP polymer detection system and visualized with WARP RED.
Flow Cytometry of Rabbit anti-Cyclin D3 antibody. Cells: mouse B cells+, 14 days post immunization (NP-CGG). Anti-Cyclin D3-RPE antibody at 1:500 for 20 min at 4°C.
Immunoprecipitation of Rabbit Anti-Cyclin D3. Lane 1: 5ug anti-cyclin D3 antibody/IP. Primary Antibody: Cyclin D3 Mouse mAb. Lane 2: 5ug anti-cyclin D3 antibody/IP. Primary Antibody: Cyclin D2 rabbit pAB. Secondary Antibody: TrueBlot IgG IP beads. Expect: ~32kDa to D3 in lane 1. No reactivity to D2 in lane 2.
ELISA results of purified Rabbit Anti-Cyclin D3 Antibody tested against BSA-conjugated peptide of cyclin D3 peptide. Each well was coated in duplicate with 0.1ug of conjugate (red line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Goat Anti-Rabbit IgG HRP conjugated (p/n 611-103-122) and TMB substrate (p/n TMBE-1000).