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Antibodies

HA Epitope Tag Antibody

Product Sizes
25 ul
600-401-384S-25UL
About this Product
SKU:
600-401-384S
Additional Names:
rabbit anti-HA epitope tag antibody, rabbit anti-hemagglutinin antibody, rabbit anti-HA tag antibody, anti-epitope
Application:
ChIP, Detection/Quantitation, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. HA tag is frequently incorporated into recombinant proteins for a variety of purposes. Rockland's anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-HA antibody was purified from whole rabbit serum prepared by repeated immunizations with the epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous pr
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Tagged Antibodies
Rockland's Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue. Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 um were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2,000 and reacted at 4° C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayer's hematoxylin. Personal Communication, Behzad Yeganeh,U. Manitoba, Winnipeg, Canada.
Anti-HA epitope tag polyclonal antibody detects HA-tagged recombinant proteins by western blot. Polyclonal Rabbit anti-HA epitope tag, at a 1:2,000 dilution, was used to detect 1.0 ug of 12-Epitope Tag Protein Marker Lysate (p/n MB-301-0100) containing the HA epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The lysate was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with Rockland's anti-HA tag antibody for 1 h at room temperature followed by washes and reaction with a 1:20,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Kinesin-5 Cut7 binds the G Gamma-TuRC MTOC.(a) Kinesin-5 and kinesin-14 constructs used in Fast Protein Liquid Chromatography. V5-tagged Cut7 and two truncation constructs were used, in addition to one FLAG-Pkl1 truncated construct that retains full Pkl1 activity. Cut7 constructs are V5-tagged full-length Cut7 (aa 1-1,085), Cut7-Head-Stalk (Cut7HS, aa 1-888) and Cut7-Stalk-Tail (Cut7-ST, aa 443-1,085). (b) Western blot profiles of whole-cell extracts fractionated by Separose 6 using FPLC. (c) Western blots of Cut7 constructs immunoprecipitated from whole-cell extracts using anti-V5 magnetic beads with empty strain negative controls. (d) Cartoon diagram of 6-His tagged Pkl1 Tail peptide co-immunoprecipitation assay using magnetic beads with His affinity and FPLC fraction 15. (e) Pkl1 Tail peptide co-immunoprecipitation of G Gamma-TuRC core subunits and V5-Cut7ST using a short Pkl1 Tail peptide (PG GammaT). Mutated peptide PG GammaM has significantly reduced interaction with the fission yeast G Gamma-TuRC. The anti-HA antibody detects the HA-tagged G Gamma-TuRC protein Alp4. Figure provided by CiteAb. Source: Nat Commun, PMID: 25348260.
SARS 3a induces NLRP3 inflammasome activation by multiple mechanisms. A) Immunoblot analysis of the pro- and cleaved forms of caspase-1 and IL-1B Beta after reconstitution of inflammasome in HEK 293T cells transfected with SARS 3a with or without NEK7 shRNA. B) Immunoblot analysis of the pro- and cleaved forms of caspase-1 and IL-1B Beta after reconstitution of inflammasome and transfection with SARS 3a or SARS 3a C133A. C) Immunoblot analysis of the pro- and cleaved forms of caspase-1 and IL-1B Beta after co-transfection with caspase-1, IL-1B Beta, and SARS 3a or SARS 3a C133A. D) Immunoprecipitation analysis of interaction between SARS 3a or SARS 3a C133A and caspase-1. All western blot data are representative of two or three independent experiments Figure provided by CiteAb. Source: Cell Death Dis, PMID: 30185776.
SARS 3a induces NLRP3 inflammasome activation by multiple mechanisms. A) Immunoblot analysis of the pro- and cleaved forms of caspase-1 and IL-1B Beta after reconstitution of inflammasome in HEK 293T cells transfected with SARS 3a with or without NEK7 shRNA. B) Immunoblot analysis of the pro- and cleaved forms of caspase-1 and IL-1B Beta after reconstitution of inflammasome and transfection with SARS 3a or SARS 3a C133A. C) Immunoblot analysis of the pro- and cleaved forms of caspase-1 and IL-1B Beta after co-transfection with caspase-1, IL-1B Beta, and SARS 3a or SARS 3a C133A. d Immunoprecipitation analysis of interaction between SARS 3a or SARS 3a C133A and caspase-1. All western blot data are representative of two or three independent experiments Figure provided by CiteAb. Source: Cell Death Dis, PMID: 30185776.