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Antibodies

Collagen Type IV Antibody

Product Sizes
25 ul
600-401-106S-25UL
About this Product
SKU:
600-401-106S
Additional Names:
COL4A1-COL4A6|rabbit anti-Collagen Type IV antibody, Arresten antibody, Canstatin antibody, Collagen Of Basement Membrane Alpha 1 Chain antibody, Collagen alpha-1 (IV) chain, COL4A1
Application:
Detection/Quantitation, Dot Blot, Electron Microscopy, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.18 mg/ml
Extra Details:
Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Collagen Type IV from human and bovine placenta
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Bovine, Human
Shipping Conditions:
Dry Ice
Specificity:
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Rockland anti collagen IV antibody (600-401-106 Lot 25440, 1:400, 45 min RT) showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli; and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA
Immunohistochemistry results of Rabbit Anti-Collagen Type IV Antibody. Tissue: human lung tissue. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type IV (p/n 600-401-106-0.1) at 1:15 for 1 hr at 37 °C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: basement membranes and vessels.
Immunohistochemistry results of Rabbit Anti-Collagen Type IV Antibody. Tissue: human skeletal muscle cells. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type IV (p/n 600-401-106-0.1) at 1:15 for 1 hr at 37 °C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: cells surrounded by collagen IV fibers.
Immunohistochemistry results of Rabbit Anti-Collagen Type IV Antibody. Tissue: human renal oncocytoma. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type IV (p/n 600-401-106-0.1) at 1:15 for 1 hr at 37 °C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: dense collagen IV positive membranes surrounding tumor cell nests.
SMFC tracking in larval newt limb regeneration.(a) Larva (3 months old). It has four limbs, as well as the gills and tail fin. Scale bar, 4 mm. (b) Monitoring of SMFCs (mCherry+) during limb regeneration (n=6). mCherry was not detected in the regenerating part of the limb until ~30 days when the amputated limb had almost been recovered (see Supplementary Movie 1). Arrowheads: flexor muscle for digits (see Fig. 2). Scale bar, 1 mm. (c) Sections of regenerating limbs (n=3 for each stage). SMFC-derived mCherry+ cells were not observed in the blastema. Lines: amputation site. m: muscle. Scale bar, 100 um. (d-f) Pax7 immunolabelling of regenerating limbs on day 12 (n=3) and (g) day 15 (n=3) after amputation. (d) On day 12, a few Pax7+ nuclei (arrowheads) were detected in blastema cells and in satellite cells along the muscle fibres. Col IV, collagen type IV immunoreactivity. DAPI (4,6-diamidino-2-phenylindole), nuclei. Scale bar, 300 um. The Pax7+ nuclei pointed by arrowheads were enlarged in e and f, respectively. Scale bars, 100 um. (g) On day 15 when the regenerating part of the limb grew more distally, the number of Pax7+ nuclei (arrowheads) in the blastema was dramatically increased. Scale bar, 100 um. (h) Summary. In larval newts, MPCs, potentially satellite cells, were recruited for new muscle during limb regeneration, whereas SMFCs were not. Figure provided by CiteAb. Source: Nat Commun, PMID: 27026263.