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Antibodies

DR3 Antibody

Product Sizes
100 ug
200-401-AX3-100UG
About this Product
SKU:
200-401-AX3
Additional Names:
DR3 Antibody, DR3, TR3, DDR3, LARD, APO-3, TRAMP, WSL-1, WSL-LR, TNFRSF12, APO3, DR3, WSL, WSL1, UNQ455/PRO779, Tumor necrosis factor receptor superfamily member 25, Apo-3|TNFRSF25
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD1-5. The ligand for this novel death receptor has been defined as TWEAK, also termed Apo3L. DR3 is highly expressed in the tissues enriched in lymphocytes including PBL, thymus and spleen. Like TNFR1, DR3 induces apoptosis and NF-kB activation.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-DR3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids in extracellular domain of human DR3 precursor.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-DR3 Antibody is affinity chromatography purified via peptide column. Cross reactivity with DR3 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunocytochemistry of Rabbit anti-DR3 antibody. Cell Type: Jurkat cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-DR3 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: DR3 is membrane bound. Staining: DR3 as precipitated brown signal with hematoxylin purple nuclear counterstain.
Immunofluorescence Microscopy of Rabbit anti-DR3 antibody. Cell TYpe: Jurkat cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: anti-DR3 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: DR3 is membrane bound. Staining: DR3 as red fluorescent signal with bis-benzimide (blue) nuclear counterstain.
Western Blot of Rabbit anti-DR3 antibody. Lane 1: molecular weight marker. Lane 2: Jurat total cell lysate. Load: 35 ug per lane. Primary antibody: DR3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45.4 kDa, 60 kDa for DR3. Other band(s): DR3 splice variants and isoforms.