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Antibodies

p53 Antibody

Product Sizes
100 ug
200-301-174-100UG
About this Product
SKU:
200-301-174
Additional Names:
mouse anti-p53 antibody, mouse anti-Tumor Suppressor p53 antibody, Phosphoprotein p53 antibody, TP53 antibody, Transformation related protein 53 antibody, TRP53 antibody, cellular Tumor antigen p53 antibody|TP53
Application:
ChIP, Detection/Quantitation, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride
CE/IVD:
RUO
translate.label.attr.clone:
BP53-12
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
The p53 gene like the Rb gene, is a tumor suppressor gene. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism; its activity stops the formation of tumors. If a person inherits only one functional copy of the p53 gene from their parents, they are pre-disposed to cancer and usually develop several independent tumors in a variety of tissues in early adulthood. This condition is rare, and is known as Li-Fraumeni syndrome. However, mutations in p53 are found in most tumor types, and so contribute to the complex network of molecular events leading to tumor formation. Anti-p53 Antibody is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, JNK/SAPK Pathway, Metabolic Disorder, Neurobiology, and p38 Pathway research.
Formulation:
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Host:
Mouse
Immunogen:
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.
Isotype:
IgG2a, IgG2a, kappa
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This protein A purified mouse monoclonal antibody reacts specifically with p53 in human tissues and cell lines. The antibody recognizes a 53 kDa band corresponding to p53. Cross reactivity with p53 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Monoclonal Antibody
Immunofluorescence microscopy of HeLa cells using anti-p53. Rockland's Protein A purified Mab anti-p53 was used at a 1:100 dilution in 10% normal goat serum in PBS and reacted overnight at 4° C. After washes cells were incubated with a 1:500 dilution of AlexaFluor594 Goat-a-Mouse IgG diluted in normal goat serum for 1 h at room temperature. Personnel Communication. Kuldeep Patel, Loyola University.
Western blotting using anti-p53 Antibody. Lane 1: HeLa whole cell lysate (p/n W09-000-364), Lane 2: HeLa cytosol fraction, and Lane 3: HeLa nuclear extract (p/n W09-001-367). Load of 15 ug was separated by 10% SDS-PAGE and transferred to nitrocellulose membrane. The membrane was blocked with 3% milk/TBST for 1 h at room temperature followed by incubation with Rockland's Protein A purified Mab anti-p53 antibody overnight at 4° C diluted 1:1,500 in blocking solution. The membrane was washed 3X with TBST and then incubated with a 1:2,000 dilution of HRP Goat-a-Mouse IgG (p/n 610-103-121) diluted in blocking buffer for 1 h at room temperature. After final washes the proteins reactive on the membrane were detected using ECL. Other detection systems will yield similar results. Personnel Communication. Kuldeep Patel, Loyola University.
Immunofluorescence of Mouse anti-p53 antibody. Tissue: human brain. Fixation: free-floating. Antigen retrieval: not required. Primary antibody: anti-human-p53 antibody at 1:500 for 1 h at RT. Co-stained with YFP and Sox2 antibodies. Localization: p53 is nuclear and cytoplasmic. Staining: p53 as precipitated blue with Cy5, YFP as precipitated green with Cy2, and Sox2 as precipitated red with Cy3. z-stacks from confocal expressed as one composite focal plain.