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Antibodies

Ash2 Antibody

Product Sizes
100 ul
100-401-V87-100UL
About this Product
SKU:
100-401-V87
Additional Names:
ASH2L|Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein, ASH2L1, ASH2L2, Bre2
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
None
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Ash2 is a component of the Set1/Ash2 histone methyltransferase (HMT) complex. This complex specifically methylates K4 of histone H3, thereby activating transcription. Methylation of K4 is blocked by premethylation of the neighboring K9, a repressor of transcription. This indicates that the Set1/Ash2 HMT complex mediates the crosstalk between K9 methylation and K4 methylation. Ash2 plays a role in hematopoiesis and may be associated with some kinds of leukemia. Anti-Ash2 Antibody is ideal for research in Gene Expression, Epigenetics, Cell Signaling and Cancer.
Format:
Antiserum
Host:
Rabbit
Immunogen:
Anti-Ash2 Antibody was produced in rabbits by repeated immunizations with three different synthetic peptides of mouse Ash2: two containing an amino acid sequence from the internal and one containing an amino acid sequence from the C-terminal part of the protein.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-Ash2 Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. Cross reactivity with Ash2 from other species not tested.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
ELISA results of Rabbit anti-Ash2 antibody. Antigen: BSA conjugated Ash2. Coating amount: 0.1 ug per well. Dilution series: serial dilution. Estimated Antibody Titer to be 1:24,000. Substrate: TMB (p/n TMBE-1000).
Immunofluorescence Microscopy results of Rabbit anti-Ash2 antibody. Tissue: NIH3T3 cells. Fixation: 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Antigen retrieval: not required. Primary antibody: Ash2 antibody at 1:200 for 1 h at RT. Secondary antibody: Alexa488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Ash2 is nuclear and occasionally cytoplasmic. Staining: Ash2 as green fluorescent signal with DAPI (blue) nuclear counterstain.
Western Blot results of Rabbit anti-Ash2 antibody. (Image A.) Lane 1: (3T3) NIH3T3 lysates. Lane 2: (E14) embryonic stem cell E14Tg2a lysates. Lane 3 (M): Ladder. Primary antibody (A): Ash2 antibody at 1:1000 for overnight at 4°C. (Image B.) Lane 1 (M): Ladder. Lane 2: (NS) mouse neural stem cell lysates transfected with GFP tagged Ash2. Primary antibody (B): Ash2 antibody at 1:500 for overnight at 4°C. Secondary antibody: goat anti-rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBS-Tween overnight at 4°C. Predicted: 68kDa endogenous, 106kDa GFP tagged Ash2.