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Proteins and Peptides

Ostreolysin (Cytolysin)

Product Sizes
50 ug
£301.18
500-076-50UG
100 ug
£411.76
500-076-100UG
About this Product
SKU:
500-076
Additional Names:
Ostreolysin A6
translate.label.attr.clone:
(#6C12)
Extra Details:
Ostreolysin, is a cytosolic protein of 15 kDa pore-forming protein from the edible oyster mushroom (Pleurotus ostreatus), is lytic to membranes containing both cholesterol and sphingomyelin. Its cytotoxicity to Chinese hamster ovary cells correlates with their cholesterol contents and with the occurrence of ostreolysin in the cells detergent resistant membranes. Moreover, ostreolysin binds to supported monolayers and efficiently permeabilizes sonicated lipid vesicles, only if cholesterol is combined with either sphingomyelin or dipalmitoyl-phosphatidylcholine. Addition of mono- or di-unsaturated phosphatidylcholine to the cholesterol/sphingomyelin vesicles dramatically reduces the ostreolysin's activity. It appears that the protein recognizes specifically a cholesterol-rich lipid phase, probably the liquid-ordered phase. Ostreolysin was purified by combination of ion-exchange and size-exclusion chromatography.
Formulation:
lyophilized
Host:
HEK293
Molecular Weight:
14.9 kDa
Purity:
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Reactivities:
Yeast/Fungi
Sequence:
AYAQWVIIIIHNVGSQDVKIKNLKASWGKLHADGDKDAEVSASNYEGKIVKPDEKLQINACGRSDAAEGTTGTFDLVDPADGDKQVRHFYWDCPWGSKTNTWTVSGSNTKWMIEYSGQNLDSGALGTITVDTLKKGN
Shipping Conditions:
Ambient
Storage Conditions:
Room Temperature
Supplier:
ReliaTech
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins