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Proteins and Peptides

Leptin pegylated super antagonist (mutant D23L/L39A/D40A/F41A)

Product Sizes
50 ug
£404.71
500-051-50UG
100 ug
£674.12
500-051-100UG
About this Product
SKU:
500-051
Additional Names:
Lep; ob; obese
translate.label.attr.clone:
(#12G3)
Extra Details:
Mono-pegylated mouse super leptin antagonist (with 20 kDa PEG) is one polypeptide chain containing 146 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 35.6 kDa as determined by MS. However due to enlarged hydrodymanic volume pegylated recombinant super mouse leptin antagonist runs on the SDS-PAGE as a 55 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. Pegylated recombinant super mouse leptin antagonist half-life in circulation after SC injection was over 20 hours. Super mouse leptin antagonist (SMLA) was initially mutated, resulting in D23L/L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques. Its pegylation is similar to that described in Elinav et al. Endocrinology 150:3083-91 (2009) for pegylated recombinant mouse leptin antagonist.
Formulation:
lyophilized
Host:
Insect/Arthropod
Molecular Weight:
35.6 KkDa
Purity:
> 95.0% as determined by Gel filtration and SDS-PAGE gel.
Reactivities:
Mouse
Sequence:
AVPIQKVQDDTKTLIKTIVTRINLISHTQSVSAKQRVTGAAAIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAFSKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDVSPEC
Shipping Conditions:
Ambient
Storage Conditions:
Room Temperature
Supplier:
ReliaTech
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins