AAV9 Xpress ELISA
Product Sizes
96 tests
£843.00
PRAAV9XP-96TESTS
About this Product
- SKU:
- PRAAV9XP
- Application:
- ELISA
- CE/IVD:
- RUO
- Extra Details:
- Fast, easy and reliable AAV9 titer determination with PROGEN's AAV9 Xpress ELISA: assay time was reduced to less than 2 hours by adjustment of the kit components and the protocol of our standard AAV9 Titration ELISA seamless and risk-free transition from our standard AAV9 Titration ELISAs (PRAAV9) to the Xpress version since the composition of the kit's core characteristics are not changed same accuracy and reproducibility as the standard AAV9 Titration ELISAs Adeno-associated viruses (AAV) are nonpathogenic ssDNA viruses, which are a subject of intense studies as viral vectors for gene therapy. The virus transduces a variety of dividing and nondividing cells showing long-term gene expression with low cellular immune response. AAV has been used in several clinical trials (e.g. FIX, CFTR, Parkinson's, Canavan disease) showing no serious vector-related adverse effects. AAV9 is especially useful for applications in the CNS. Methods for the characterization of AAV preparations currently include titration ELISA, real-time PCR, DNA dot blot, determination of transducing units, infectious center assay, SDS-PAGE or electron microscopy. AAV preparations contain up to 10fold more empty virus capsids than filled, therapeutically active virus particles. Since both full and empty particles can induce an immune reaction, it is crucial for in in vivo appilcations to determine the complete particle titer. Immunotitration by PROGEN's AAV9 Titration ELISA offers a fast, sensitive and reproducible method for titration of intact AAV9 wt virions, AAV9 recombinant virions or assembled and intact empty AAV9 capsids. The ELISA principle: The assay is based on the sandwich ELISA technique where a monoclonal antibody (mab) specific for a conformational epitope on assembled AAV capsids is coated onto the plate and is used to capture AAV particles from the specimen. The detection of captured AAV particles is a two-step process.
- A biotin-conjugated mab is bound to the captured AAV particles. A streptavidin peroxidase conjugate reacts with the biotin molecules. The addition of the substrate results in a color reaction which is proportional to the amount of specifically bound viral particles.
- Format:
- Reagent kit, 12 x 8-well strips
- Reactivities:
- Virus
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Progen
- Type:
- Assay Kits: ELISAs
- Manufacturer's Data Sheet:PRAAV9XP







