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Antibodies

anti-Glial Filament Protein, guinea pig polyclonal antibody

Product Sizes
100 ul
£364.00
GP52-100UL
About this Product
SKU:
GP52
Additional Names:
Glial fibrillary acidic protein, GFAP, GFAP
Application:
Immunohistochemistry
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Specific detection of GFAP (Glial Fibrillary Acidic Protein, Glial Filament Protein) (Mr 52000 polypeptide). Tumors specifically detected: Astrocytomas, gangliomas, meulloblastomas, certain teratomas
Formulation:
Contains 0.09% sodium azide and 0.5% BSA
Host:
Guinea Pig
Immunogen:
Gliafilament protein purified from bovine spinal cord
Purification:
Stabilized antiserum
Reactivities:
Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:GP52
IHC analysis of mouse brain using anti-glial fibrillary acidic protein antibody (Cat. No. GP52). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-GFAP (Cat. No. GP52) was diluted in PBS (1:2000) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratries) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The picture was acquired using microscopy (courtesy of J.Hess
University Hospital Heidelberg).
IHC analysis of mouse spinal cord using anti-glial fibrillary acidic protein antibody (Cat. No. GP52). IHC was performed on formalin fixed frozen sections (12 um). Heat induced antigen retrieval was performed in citrate buffer pH 6.0 for 1 h at 65°C. After preparation the tissue was blocked with 5% donkey serum in fish skin buffer for 1 h at RT. The primary antibody anti-GFAP (Cat. No. GP52) was diluted in fish skin buffer (1:500) and incubated at RT over-night. The secondary antibody donkey anti-guinea pig Alexa488 was also diluted in fish skin buffer (1:1