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Antibodies

anti-Perilipin 2 (C-terminus), guinea pig polyclonal antibody

Product Sizes
100 ul
£384.00
GP41-100UL
About this Product
SKU:
GP41
Additional Names:
Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Adipophilin/ ADRP/ PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see Heid et al. 1998 & 2013; for review see Targett-Adams et al. 2003); inducible by etomoxir. Enhanced expression of Adipophilin/ ADRP/ PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry via lipid droplets (see Hope et al. 2002). Polypeptide reacting: Adipophilin/ ADRP/ PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72 Tissue Immunolocalization: Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages. Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, e63061 (2013). Targett-Adams, P. et al. Live Cell Analysis and Targeting of the Lipid Droplet-binding Adipocyte Differentiation-related Protein. J. Biol. Chem. 278, 15998-16007 (2003). Hope, R. G., Murphy, D. J. & McLauchlan, J. The Domains Required to Direct Core Proteins of Hepatitis C Virus and GB Virus-B to Lipid Droplets Share Common Features with Plant Oleosin Proteins. J. Biol. Chem. 277, 4261-4270 (2002). Heid, H. W., Moll, R., Schwetlick, I., Rackwitz, H. R. & Keenan, T. W. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res. 294, 309-21 (1998).
Formulation:
Contains 0.09% sodium azide
Host:
Guinea Pig
Immunogen:
Synthetic peptide (C-terminal aa 423 - 437 of human adipophilin)
Purification:
Stabilized antiserum
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Polyclonal Antibody
  • Manufacturer's Data Sheet:GP41
Western blot analysis of HepG2 cell lysate with anti-Perilipin 2 antibody. Western blot analysis was performed on 30 ug HepG2 lysate and 30 ug HepG2 + OA lysate. Cells were previously treated with 400 uM oleic acid (OA) if indicated. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibodies anti-Perilipin 2 (C-terminus) guinea pig polyclonal (Cat. No. GP41) and anti-GAPDH were diluted in blocking buffer (1:500 anti-Perilipin
1.2 ug/ml anti-GAPDH
Cat. No. 690975) and incubated at 4°C over-night. The secondary antibodies donkey anti-guinea pig A647 and goat anti-mouse A488 were also diluted in blocking buffer (both 1:300) and incubated for 1 h at RT. The bands were visualized by fluorescent detection.
Immunofluorescence analysis of HepG2 cells with anti-Perilipin 2 antibody. HepG2 cells were analysed either untreated or treated with 400 uM oleic acid over-night to incorporate lipid droplets. Fixation was performed using 3% paraformaldehyde for 10 min at RT. Cells were blocked with 5% BSA in PBST for 1 h at RT and permeabilized with 0.3% Triton-x 100 in PBS for 10 min at RT. The primary antibody anti-Perilipin 2 (C-terminus) guinea pig polyclonal (Cat. No. GP41) was diluted in blocking buffer (1:100) and incubated over-night at 4°C. The secondary antibody donkey anti-guinea pig AF647 was also diluted in blocking buffer (antibody concentration 3.75 ug/ml) and incubated for 30 min at 37°C and 30 min at RT. DNA was stained with DAPI in blue.
WB with anti-Perilipin 2 antibody (Cat. No. GP41