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Proteins and Peptides

Keratin K8, human, rekombinant, MW 53,554

Product Sizes
100 ug
£312.00
62213-100UG
About this Product
SKU:
62213
Additional Names:
Cytokeratin 8
CE/IVD:
RUO
Extra Details:
Protein standard for immunoblotting, immunization and immunoassays. Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS). - Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841 - Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255
Purity:
> 95% (determined by SDS gelelectrophoresis)
Shipping Conditions:
Blue Ice
Source:
Human recombinant, produced in E. coli
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Recombinant Proteins
  • Manufacturer's Data Sheet:62213