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Antibodies

Anti-B Beta-Tubulin Antibody

Product Sizes
100 ul
£372.00
TM1541-100UL
About this Product
SKU:
TM1541
Additional Names:
TUBB3
Application:
ELISA, Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
M154
Clonality:
Monoclonal
Extra Details:
Our B Beta-Tubulin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat B Beta-Tubulin and is Protein A purified. It is great for use in WB, ICC., ECM
Format:
Protein A Purified
Formulation:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gene Details:
TUBB3
Host:
Mouse
Immunogen:
Clone (M154) was generated from purified porcine brain tubulin.
Isotype:
IgG1
Molecular Weight:
50
Physical State:
Liquid
Purification:
Protein A purified culture supernatant.
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
This antibody detects a 50 kDa* protein corresponding to the molecular mass of B Beta-Tubulin on SDS-PAGE immunoblots of purified brain tubulin, mouse brain tissue, rat PC12 cells, and human A431 and SH-SY5Y cells.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-B Beta-Tubulin (a.a. 168-177) (lanes 1 & 2), anti-B Beta-Tubulin (Ser-172) (lanes 3 & 4), and anti-B Beta-Tubulin (TM1541) (lanes 5 & 6).
Immunocytochemical labeling in C2C12 cells using anti-B Beta-Tubulin (TM1541) monoclonal antibody and anti-B Beta-Tubulin (Ser-172) polyclonal antibody. The specificity of the binding for the latter antibody was demonstrated by using the antibody in the presence of phospho-B Beta-Tubulin (Ser-172) peptide (TX1725).