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Antibodies

Anti-Phosphoserine/threonine Antibody

Product Sizes
100 ul
£433.00
PP2551-100UL
About this Product
SKU:
PP2551
Additional Names:
Phosphoser/thr mAb
Application:
ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
pSer/Thr - Phosphorylated
Extra Details:
Our Anti-Phosphoserine/threonine rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Anti-Phosphoserine/threonine and is antigen affinity purified. It is great for use in WB, ICC, IP., ECM
Format:
Antigen Affinity Purified
Formulation:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gene Details:
N/A
Host:
Rabbit
Immunogen:
Anti-Phosphoserine/threonine was generated from a panel of phosphoserine and phosphothreonine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved in many species.
Isotype:
IgG
Molecular Weight:
N/A
Physical State:
Liquid
Purification:
Affinity purified rabbit serum
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
This antibody was cross-adsorbed to unphosphorylated peptide then affinity purified using a mix of phosphoserine and phosphothreonine peptides (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Bar graph showing anti-Phosphoserine/threonine (PP2551) binding to a variety of phosphoserine and phosphothreonine peptides, but not control peptide containing unphosphorylated serine or phosphotyrosine.
Western blot showing N2B-Titin total phosphorylation (cat. PP2551, 1:500) in male and female mRen2 and WT rats. Image from publication CC-BY-4.0. PMID: 37363100
Western blot image of plant extracts with intact FonKin4 and truncated S_TKc domain-containing fragment FonKin4-ST show Ser/Thr protein kinase activity detected by anti-phospho Ser/Thr antibody (Cat no PP2551). FonKin4 is a Ser/Thr protein kinase and its S_TKc domain is sufficient for its pathogenicity function in Fon. Image from publication CC-BY-4.0. PMID: 38690366.
Purified His-tagged NFR1, NFR1Y429F, or NFR1T481A CDs were incubated with ATP for 30 min. The proteins were detected by immunoblotting with anti-His antibody. Phosphorylated products were detected using anti-pSer/Thr (cat. PP2551) or anti-pTyr antibodies. Un-phosphorylated NFR1-CD band is indicated by the red triangle. Image from publication CC-BY-4.0. PMID: 38670968
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.