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Antibodies

Anti-Phosphoserine/threonine Antibody

Product Sizes
100 ul
£433.00
PM3801-100UL
About this Product
SKU:
PM3801
Additional Names:
Phosphoser/thr mAb
Application:
ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
M380A/M380B
Clonality:
Monoclonal
Conjugate:
pSer/Thr - Phosphorylated
Extra Details:
Our Anti-Phosphoserine/threonine mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Anti-Phosphoserine/threonine and is Protein A purified. It is great for use in WB, ICC, IP., ECM
Format:
Protein A Purified
Formulation:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gene Details:
N/A
Host:
Mouse
Immunogen:
PM3801 Phosphoserine/threonine is a mix of two clones: Clone M380A was generated from a phosphothreonine synthetic peptide (coupled to carrier protein) and Clone M380B was generated.from a phosphoserine synthetic peptide (coupled to carrier protein).
Isotype:
IgG1
Molecular Weight:
N/A
Physical State:
Liquid
Purification:
Protein A purified culture supernatant.
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Immunocytochemical labeling of phosphoserine and phosphothreonine in control and calyculin A-treated A431 cells. The cells were labeled with mouse monoclonal anti-Phosphoserine/threonine (PM3801) and rabbit polyclonal anti-Phosphoserine/threonine (PP2551), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
WT (PKY2255) and pda1D Delta (PKY2256) cells expressing Qcr2-GFP were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with A Alpha-GFP antibody. The immunoprecipitates were then probed with A Alpha- phosphoamino acid antibody (cat. PM3801, top panel) and A Alpha-GFP antibody (bottom panel). Right panel: day 5 samples were treated overnight with lambda phosphatase or mock-treated. Image from publication CC-BY-4.0. PMID: 37454238
WT (PKY1089) and pda1D Delta (PKY1093) cells expressing Mdh1-GFP, as well as the indicated plasmids, were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with A Alpha-GFP antibody. Phosphorylation of Mdh1-GFP was detected using A Alpha-phosphoamino acid antibody (cat. PM3801), and total GFP signal in the immunoprecipitate was observed by immunoblotting with the A Alpha-GFP antibody. ϕ denotes an empty vector Image from publication CC-BY-4.0. PMID: 37454238
Mutant lat1D Delta cells (PKY2298) expressing the indicated plasmids, as well as Mdh1-GFP, were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with with A Alpha-GFP antibody. Phosphorylation of Mdh1-GFP was detected using A Alpha-phosphoamino acid antibody (cat. PM3801), and total GFP signal in the immunoprecipitate was observed by immunoblotting with the A Alpha-GFP antibody. ϕ denotes an empty vector. Image from publication CC-BY-4.0. PMID: 37454238
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).