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Antibodies

Anti-ERK1/2 (MAPK) (Thr202/Tyr204) Antibody

Product Sizes
25 ul
£289.00
p160-2024-25UL
100 ul
£552.00
p160-2024-100UL
About this Product
SKU:
p160-2024
Additional Names:
ERK 1 antibody, ERK 2 antibody, ERK-2 antibody, ERK1 antibody, erk1/2 antibody, ERK2 antibody, ERT1 antibody, ERT2 antibody, Extracellular signal regulated kinase 1 antibody, Extracellular signal-regulated kinase 2 antibody, MAP kinase 1 antibody, MAP kinase 2 antibody, MAP kinase isoform p42 antibody, MAP kinase isoform p44 antibody, MAPK 1 antibody, MAPK 2 antibody, MAPK 3 antibody, Mapk1 antibody, MAPK2 antibody, MAPK3 antibody, Mitogen-activated protein kinase 1 antibody, Mitogen-activated protein kinase 2 antibody, MK01_HUMAN antibody, p38 antibody, p40 antibody, p41 antibody, p42-MAPK antibody, PRKM 2 antibody
Application:
ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
Buffer:
10 mM HEPES (
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Thr202, Tyr204 - Phosphorylated
Extra Details:
Our Anti-ERK1/2 (MAPK) (Thr202/Tyr204) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat ERK1/2 (MAPK) (Thr202/Tyr204) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC., Phosphosolutions
Format:
Antigen Affinity Purified from Pooled Serum
Formulation:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
Gene Details:
MAPK1/2
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 of rat ERK/MAPK conjugated to keyhole limpet hemocyanin (KLH).
Isotype:
IgG
Molecular Weight:
42/44
Physical State:
Liquid
Purification:
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Reactivities:
Amphibian, Avian, Bovine, Canine, Fish, Human, Mouse, Non-human Primate, Rat
Shipping Conditions:
Blue Ice
Specificity:
Specific for endogenous levels of the ~42 - 44 kDa ERK/MAPK protein phosphorylated at Thr202 and Tyr204. Immunolabeling is completely eliminated by treatment with L Lambda-phosphatase.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunolabeling of cultured mouse hippocampal neurons fixed and stained with anti-phospho-ERK/MAPK Thr202/Tyr204 (p160-2024, green, 1:100) and red nuclear stain Propidium Iodide. The labeling identifies an increase in ERK/MAPK phosphorylation when hippocampal neurons are treated with a specific ASIC1a activator, MitTx toxin (20 nM, 4 min). Image kindly provided by Carina Weissmann, IFIBYNE-CONICET.
Immunostaining of granule cells in the dentate gyrus of the hippocampus from saline treated mouse brain staining ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, 1:500, red). The blue is staining nuclei with DAPI. The MAPK positive neurons show punctate staining primarily localized in the nucleus with few staining both cytoplasmic and nuclear. Photo courtesy of Robert Wine.
Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.
Western blot of human T47D cell lysate showing specific immunolabeling of ~42-44 kDa ERK/MAPK protein phosphorylated at Thr202/Tyr204 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (L Lambda-Ptase, 1200 units for 30 min).