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Antibodies

Anti-NCC (Thiazide sensitive NaCl cotransporter) (Thr53) Antibody

Product Sizes
25 ul
£289.00
p1311-53-25UL
100 ul
£552.00
p1311-53-100UL
About this Product
SKU:
p1311-53
Additional Names:
FLJ96318 antibody, Na Cl cotransporter antibody, Na Cl symporter antibody, Na-Cl symporter antibody, NaCl electroneutral thiazide sensitive cotransporter antibody, NCCT antibody, SLC12A3 antibody, S12A3_HUMAN antibody, slc12a3 antibody, Solute carrier family 12 (sodium/chloride transporters) member 3 antibody, Solute carrier family 12 member 3 antibody, Thiazide sensitive Na Cl cotransporter antibody, Thiazide sensitive sodium chloride cotransporter antibody, Thiazide-sensitive sodium-chloride cotransporter antibody, TSC antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
10 mM HEPES (
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Thr53 - Phosphorylated
Extra Details:
Our Anti-NCC (Thiazide sensitive NaCl cotransporter) (Thr53) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NCC (Thiazide sensitive NaCl cotransporter) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC., Phosphosolutions
Format:
Antigen Affinity Purified from Pooled Serum
Formulation:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
Gene Details:
SLC12A3
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 of mouse NCC, conjugated to keyhole limpet hemocyanin (KLH).
Isotype:
IgG
Molecular Weight:
160
Physical State:
Liquid
Purification:
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Reactivities:
Guinea Pig, Hamster, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Specific for endogenous levels of the ~160 kDa NCC protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-phosphatase.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (L Lambda-Ptase, 1200 units for 30 min).
Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (cat. p1311-53, red, 1:100,000) on the left and the absence of staining in the KO on the right. (Image courtesy of Lauren Miller, Ellison Lab, OHSU.)
Immunostaining of mouse kidney sections from control (CTL) and BRL37344 treated (BRL, 1 mg/kg) specifically labeling localized phosphorylated NCC thr53 (Cat p1311-53, green) and counterstained with Evans Blue (in red). Image from publication. CC-BY-4.0, PMID: 38652212
Two Western blots show the abundance of pNCCT53 (cat. p1311-53, 1:200) and tNCC in male Kcnj10fl/fl mice and in Ks-Kir4.1-KO mice treated with vehicle (control) and FK506 (0.75 mg/kg BW). Image from publication CC-BY-4.0. PMID: 36821372