Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Anti-Aquaporin 2 (Ser269) Antibody

Product Sizes
25 ul
£289.00
p112-269-25UL
100 ul
£552.00
p112-269-100UL
About this Product
SKU:
p112-269
Additional Names:
ADH water channel antibody, AQP 2 antibody, AQP CD antibody, AQP-2 antibody, AQP-CD antibody, AQP2 antibody, AQP2_HUMAN antibody, AQPCD antibody, Aquaporin 2 collecting duct antibody, Aquaporin CD antibody, Aquaporin-2 antibody, Aquaporin-CD antibody, Aquaporin2 antibody, Aquaporine 2 antibody, Collecting duct water channel protein antibody, MGC34501 antibody, Water channel aquaporin 2 antibody, Water channel protein for renal collecting duct antibody, WCH CD antibody, WCH-CD antibody, WCHCD antibody
Application:
ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
10 mM HEPES (
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Ser269 - Phosphorylated
Extra Details:
Our Anti-Aquaporin 2 (Ser269) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Aquaporin 2 (Ser269) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC, IP., Phosphosolutions
Format:
Antigen Affinity Purified from Pooled Serum
Formulation:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
Gene Details:
AQP2
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269 of rat aquaporin 2, conjugated to keyhole limpet hemocyanin (KLH).
Isotype:
IgG
Molecular Weight:
29/37
Physical State:
Liquid
Purification:
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Reactivities:
Bovine, Canine, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Specific for endogenous levels of the ~29 kDa AQP2 protein phosphorylated at Ser269. Also recognizes the glycosylated form of AQP2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. No. p112-269, red, 1:1000). Nuclei labeled with DAPI. Magnification 10x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
Immunolabeling of vasopressin treated mouse kidney (inner medulla) showing specific labeling of the AQP2 protein phosphorylated at Ser269 (Cat. No. p112-269, red, 1:1000) and total AQP2 (green). Nuclei labeled with DAPI. Magnification 600x. Image kindly provided by Juan Pablo Arroyo Ornelas, Vanderbilt University.
Western analysis of rat kidney inner medullary (IM) lysate from ddAVP and ddAVP + ADM-treated IM probed for total (bottom) and pSer269 (cat. p112-269, 1:1000)(top) AQP2. Brackets indicate the glycosylated AQP2 protein between 35 and 45 kDa, and the arrow indicates the un glycosylated AQP2 protein at 29 kDa. The matched pair comparisons were achieved by comparing Lane 1 with Lane 5; Lane 2 with Lane 6; Lane 3 with Lane 7; and Lane 4 with Lane 8. Image from publication CC-BY-4.0. PMID: 37047509
Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser269 in the vasopressin (AVP) treated lane (+), but not in the control lane (-).