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Antibodies

Anti-CREB (Ser133) Antibody

Product Sizes
25 ul
£289.00
p1010-133-25UL
100 ul
£552.00
p1010-133-100UL
About this Product
SKU:
p1010-133
Additional Names:
Active transcription factor CREB antibody, cAMP response element binding protein 1 antibody, cAMP response element binding protein antibody, cAMP responsive element binding protein 1 antibody, cAMP-responsive element-binding protein 1 antibody, CREB antibody, CREB-1 antibody, CREB1 antibody, CREB1_HUMAN antibody, Cyclic AMP-responsive element-binding protein 1 antibody, MGC9284 antibody, OTTHUMP00000163864 antibody, OTTHUMP00000163865 antibody, OTTHUMP00000206660 antibody, OTTHUMP00000206662 antibody, TTHUMP00000206667 antibody, Transactivator protein antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
10 mM HEPES (
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Ser133 - Phosphorylated
Extra Details:
Our Anti-CREB (Ser133) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat CREB (Ser133) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC., Phosphosolutions
Format:
Antigen Affinity Purified from Pooled Serum
Formulation:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
Gene Details:
CREB1
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 of rat CREB, conjugated to keyhole limpet hemocyanin (KLH).
Isotype:
IgG
Molecular Weight:
45
Physical State:
Liquid
Purification:
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Reactivities:
Amphibian, Avian, Bovine, Canine, Fish, Human, Mouse, Non-human Primate, Rat, Sheep
Shipping Conditions:
Blue Ice
Specificity:
Specific for endogenous levels of the ~45 kDa CREB protein phosphorylated at Ser133. Immunolabeling is completely eliminated by treatment with L Lambda-phosphatase.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunolabeling of a section of mouse piriform cortex labeled with Anti-Phospho-Ser133 CREB (cat. p1010-133, red, 1:1000). Cell nuclei are visualized with DAPI DNA stain (blue).
Immunolabeling of mouse cerebral cortex (top) and habenula (bottom) brain sections labeled with anti-phospho-ser133 CREB (cat. p1010-133, DAB, 1:100). These images were kindly provided by Dr. Anton Reiner, Univ. of Tennessee Health Science Center (Memphis, TN).
Immunolabeling of mouse hippocampal section labeled with anti-phospho-ser133 CREB (cat. p1010-133, DAB, 1:100, top). The image on the bottom is a magnification of the dentate gyrus section of the hippocampus. These images were kindly provided by Dr. Anton Reiner, Univ. of Tennessee Health Science Center (Memphis, TN).
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~45 kDa CREB phosphorylated at Ser133 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (L Lambda-Ptase, 800 units/1mg protein for 30 min).