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Antibodies

Anti-Myosin Light Chain (N-terminal region) Antibody

Product Sizes
100 ul
£372.00
MP4201-100UL
About this Product
SKU:
MP4201
Additional Names:
MLC20, RLC-C; Mylc2c; Myl9, MLC2, MRLC1, MYRL2; MLCB; MRCL3; MRLC3; MYL2B; MYL12A, myosin
Application:
ELISA, Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Our Myosin Light Chain (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Myosin Light Chain (N-terminal region) and is antigen affinity purified. It is great for use in WB, ICC., ECM
Format:
Antigen Affinity Purified
Formulation:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gene Details:
MYL12A
Host:
Rabbit
Immunogen:
MLC synthetic peptide (coupled to KLH) corresponds to amino acid residues in the N-terminus of human myosin regulatory light chain 12A (Myl12A). This peptide sequence is highly conserved in human, rat, mouse, chicken, and zebrafish Myl12A, and in other smooth muscle and non-muscle MLCs.
Isotype:
IgG
Molecular Weight:
20
Physical State:
Liquid
Purification:
Affinity purified rabbit serum
Reactivities:
Avian, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 20 kDa* band corresponding to the molecular weight of myosin light chain in mouse C2C12, rat PC12, and human A431.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Western blot analysis of C2C12 cells untreated (lanes 1, 3, 5, & 7) or treated with Lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with monoclonal anti-MLC20 (clone MY-21) (lanes 1 & 2), polyclonal anti-MLC (Ser-19) phosho-specific (lanes 3 & 4), anti-MLC (Ser-1) phosho-specific (lanes 5 & 6), or anti-MLC (a.a. 11-22) (lanes 7 & 8) .
Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (MM3441; middle) and anti-MLC (MP4201; top left), anti-MLC (Ser-19) (MP4221; middle left) and anti-MLC (Ser-1) (MP3461; bottom left). Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594 were used for detection of primary antibodies. The overlay of staining patterns are shown to the right.