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Antibodies

Anti-Myosin IIA Heavy Chain (C-terminal region) Antibody

Product Sizes
100 ul
£372.00
MP3791-100UL
About this Product
SKU:
MP3791
Additional Names:
NMHC-IIA, MYH9, myosin heavy chain
Application:
ELISA, Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Our Myosin IIA Heavy Chain (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Myosin IIA Heavy Chain (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC., ECM
Format:
Antigen Affinity Purified
Formulation:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gene Details:
MYH9
Host:
Rabbit
Immunogen:
NMHC-IIA synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse NMHC-IIA, but is not found in NMHC-IIB and is not well conserved in NMHC-IIC.
Isotype:
IgG
Molecular Weight:
200
Physical State:
Liquid
Purification:
Affinity purified rabbit serum
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 200 kDa* protein corresponding to the apparent molecular mass of NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Myosin IIA Heavy Chain (a.a. 1936-1950), then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide (MX3795).
Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).