Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Anti-His (C-terminal) Tag Antibody

Product Sizes
100 ul
£372.00
HM0501-100UL
About this Product
SKU:
HM0501
Additional Names:
His Tag, 6x His Tag, HHHHHH
Application:
ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
M050
Clonality:
Monoclonal
Conjugate:
- GOmAb
Extra Details:
Our Anti-His (C-terminal) Tag Antibody mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects na Anti-His (C-terminal) Tag Antibody and is Protein G purified. It is great for use in WB, ICC, IHC, IP, FC., ECM
Format:
Protein G Purified
Formulation:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gene Details:
N/A
Host:
Mouse
Immunogen:
Clone (M050) was generated from a recombinant protein containing a C-terminal His-tag.
Isotype:
IgG1
Molecular Weight:
N/A
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Wide/Non-species Specific
Shipping Conditions:
Blue Ice
Specificity:
Clone M050 detects recombinant proteins with C-terminal His-tag epitope. The antibody binds 6 and 10 His-tag epitopes on the C-terminal side of a protein, but does not bind N-terminal His-tag epitopes. The antibody works for western blot, immunoprecipitation, immunocytochemistry, flow cytometry, and ELISA capture.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Western blot of human recombinant DDR1 protein with C-terminal His Tag. The blot was probed with mouse monoclonal anti-His (Cterminal) Tag (HM0501) at 1:2000 (lane 1), 1:4000 (lane 2), and 1:8000 (lane 3).
Representative Standard Curve using mouse monoclonal anti-His (C-terminal) Tag antibody (HM0501) for ELISA capture of human recombinant CD46 extracellular region with a C-terminal His-tag. Captured protein was detected using anti-CD46 antibody (CM0371) antibody followed by appropriate secondary antibody HRP conjugate.
Immunocytochemical labeling of Axl recombinant protein with C-terminal His Tag bound to THP1 aldehyde fixed cells. The cells were labeled with mouse monoclonal anti-His (C-terminal) Tag antibody (HM0501). The antibody was detected using goat anti-mouse DyLight® 594.