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Antibodies

Anti-EphA4 (Tyr-602) [conserved site], Phosphospecific Antibody

Product Sizes
100 ul
£433.00
EP2731-100UL
About this Product
SKU:
EP2731
Additional Names:
SEK, Eph
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Tyr-602 - Phosphorylated
Extra Details:
Our EphA4 (Tyr-602)) phosphospecific primary antibody from PhosphoSolutions is rabbit polyclonal. It detects chicken, human, mouse, and rat EphA4 (Tyr-602)) and is antigen affinity purified. It is great for use in WB, IHC., ECM
Format:
Antigen Affinity Purified
Formulation:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gene Details:
EPHA4
Host:
Rabbit
Immunogen:
EphA4 (Tyr-602) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding phosphorylated tyrosine 602 in human EphA4. This sequence has significant homology to the conserved site in rat and mouse EphA4, and is conserved well in most other EphA and EphB family members.
Isotype:
IgG
Molecular Weight:
120
Physical State:
Liquid
Purification:
Affinity purified rabbit serum
Reactivities:
Avian, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The purified antibody detects a 120 kDa* band corresponding to EphA4 in Western blots of HUVEC cells treated with pervanadate and in mouse brain tissue.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Representative IHC image of p-EPHA4 (cat. EP2731, 1:100) in Ptpro+/+ and Ptpro-/- mice hippocampi. Image from publication CC-BY-4.0. PMID: 37485875
Paraformaldehyde-fixed zebrafish embryos were probed with anti-EphA4 (Tyr-602) (EP2731) then detected using Alexa Fluor 647 goat anti-rabbit. Arrows show labeling of somite boundaries and the notochord. (Image provided by Dr. Scott Holley at the Department of Molecular, Cellular and Developmental Biology, Yale University.)
Immunoblotting of PTPRO, p-SRC, SRC, p-EPHA4 (cat. EP2731, 1:1000), EPHA4, p-EPHB2, and EPHB2 in Ptpro+/+ and Ptpro-/- mice hippocampi under Chemotherapy-related cognitive impairment (CRCI). Image from publication CC-BY-4.0. PMID: 37485875
Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).
Western blot image of mouse HCA-1 (left) and human Hep3B (right) cells treated with RNase1 (1 ug/ml) at the indicated time points specifically labeling EphA4 (EM2801, 1:1000) and phosphorylated EphA4-Tyr604 (EP2731, 1:1000) Image from publication CC-BY-4.0. PMID: 38307859