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Antibodies

Anti-Coronin-1B (C-terminus) Antibody

Product Sizes
100 ul
£372.00
CP2581-100UL
About this Product
SKU:
CP2581
Additional Names:
Coronin2, Coro1B
Application:
ELISA, Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Our Coronin-1B (C-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Coronin-1B (C-terminus) and is antigen affinity purified. It is great for use in WB, ICC., ECM
Format:
Antigen Affinity Purified
Formulation:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gene Details:
CORO1B
Host:
Rabbit
Immunogen:
Coronin-1B (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids at the C-terminus of human coronin-1B. This sequence has high homology to the conserved site in rat and mouse coronin-1B, and less than 50% homology to similar regions in other coronins.
Isotype:
IgG
Molecular Weight:
60
Physical State:
Liquid
Purification:
Affinity purified rabbit serum
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 60 kDa* band corresponding to Coronin-1B on SDS-PAGE immunoblots of human A431 and HeLa, mouse brain, and rabbit spleen fibroblasts.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Western blot analysis of human A431 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Coronin-1B (C-terminal region) (lanes 1 & 2) and anti-Coronin-1B (Ser-2) (lanes 3 & 4).
Immunocytochemical labeling of coronin-1B in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal Coronin-1B (C-terminus) and Coronin-1B (Ser-2) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2585 or CX2625).