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Antibodies

Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

Product Sizes
25 ul
621-GFAP-25UL
100 ul
621-GFAP-100UL
About this Product
SKU:
621-GFAP
Additional Names:
wu:fb34h11 antibody, ALXDRD antibody, cb345 antibody, etID36982.3 antibody, FLJ42474 antibody, FLJ45472 antibody, GFAP antibody, GFAP_HUMAN antibody, gfapl antibody, Glial fibrillary acidic protein antibody, Intermediate filament protein antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody
Application:
Immunocytochemistry, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
30 mg/ml
Extra Details:
Our Anti-Glial Fibrillary Acidic Protein (GFAP) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Glial Fibrillary Acidic Protein (GFAP) and is total IgY fraction. It is great for use in WB, IHC, ICC.
Format:
Total IgY fraction
Gene Details:
GFAP
Host:
Chicken/Avian
Immunogen:
Recombinant human GFAP.
Isotype:
IgY
Molecular Weight:
50
Physical State:
Liquid
Reactivities:
Bovine, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Specific for endogenous levels of the ~50 kDa GFAP protein. A lower band at ~45 kDa is a proteolytic fragment derived from the GFAP molecule.
Storage Conditions:
-20[o]C Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunolabeling of mixed neuron and glia cultures where astrocytes are strongly and specifically labeled with Anti-GFAP(cat. 621-GFAP, 1:1000, red), and nuclear staining with DAPI (blue).
Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3(cat. 583-FOX3, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (cat. 621-GFAP, 1:1000, green) and nuclear staining was done with DAPI (blue).
Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody (cat. 1520-NSE, red, 1:500) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells.
Immunostaining of E20 rat cortical neuron/glial cell culture stained with anti-vimentin (cat. 2107-VIM, red, 1:2000) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is DAPI staining nuclear DNA. Vimentin is expressed alone in fibroblastic and developing cells and appear red. The astrocytes that appear red express only GFAP, while the golden yellow and yellow astrocytes express GFAP and Vimentin.
Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP(cat. 621-GFAP, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.