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Antibodies

Anti-FOX3 (NeuN) Antibody

Product Sizes
25 ul
£289.00
583-FOX3-25UL
100 ul
£552.00
583-FOX3-100UL
About this Product
SKU:
583-FOX3
Additional Names:
FLJ56884 antibody, FLJ58356 antibody, Fox-1 homolog C antibody, fox1 homolog C antibody, Fox 3 antibody, FOX3NeuN antibody, hexaribonucleotide binding protein 3 antibody, HRNBP3 antibody, NeuN antibody, NEUN antibody, neuronal nuclei antibody, Rbfox3 antibody, RFOX3_HUMAN antibody, RNA binding protein fox-1 homolog 3 antibody, RNA binding protein fox 1 homolog (C. elegans) 3 antibody
Application:
ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
1B7
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Our Anti-FOX3 (NeuN) primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, human, mouse, and rat FOX3 (NeuN) and is Protein G purified. It is great for use in WB, IHC, ICC., Phosphosolutions
Format:
Protein G Purified
Formulation:
PBS + 50% glycerol and 5 mm NaN3
Gene Details:
RBFOX3
Host:
Mouse
Immunogen:
Recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E. Coli.
Isotype:
IgG2b
Molecular Weight:
46/48
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Bovine, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Specific for endogenous levels of the ~46/48 kDa FOX3 protein doublet.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3(cat. 583-FOX3, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (cat. 621-GFAP, 1:1000, green) and nuclear staining was done with DAPI (blue).
Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3(cat. 583-FOX3, green, 1:1000) and Anti-MAP2 (cat. 1100-MAP2, red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
Cell Line: human-derived iPSC cerebral organoids. Neurofilament-H (green), NeuN (purple), DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
Cell Line: human-derived iPSC cerebral organoids. MAP2 (pink), NeuN (yellow), DAPI (turquoise). Image courtesy of Caroline Krall, Johns Hopkins University
Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (cat. 1453-NFL, 1:2000, green) and labeling of FOX3/NeuN (cat. 583-FOX3, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.