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Antibodies

Anti-Vimentin Antibody

Product Sizes
25 ul
£289.00
2107-VIM-25UL
100 ul
£552.00
2107-VIM-100UL
About this Product
SKU:
2107-VIM
Additional Names:
CTRCT30 antibody, Epididymis luminal protein 113 antibody, FLJ36605 antibody, HEL113 antibody, VIM antibody, VIME_HUMAN antibody, Vimentin antibody
Application:
ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
2D1
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
Our Anti-Vimentin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Vimentin and is Protein G purified. It is great for use in WB, IHC, ICC., Phosphosolutions
Format:
Protein G Purified
Formulation:
PBS + 10 mM NaN3.
Gene Details:
VIM
Host:
Mouse
Immunogen:
Recombinant human vimentin purified from E. coli.
Isotype:
IgG2a
Molecular Weight:
50
Physical State:
Liquid
Purification:
Protein G Purified
Reactivities:
Amphibian, Avian, Bovine, Canine, Feline, Fish, Goat, Guinea Pig, Hamster, Horse, Human, Insect/Arthropod, Mouse, Non-human Primate, Other Mammal, Other Species, Rabbit, Rat, Sheep
Shipping Conditions:
Blue Ice
Specificity:
Specific for endogenous levels of the ~50 kDa vimentin protein.
Storage Conditions:
-20[o]C Aliquot. Avoid freeze/thaw cycles. Store undiluted., 2-8[o]C Aliquot. Avoid freeze/thaw cycles. Store undiluted.
Type:
Antibody: Monoclonal Antibody
Immunostaining of E20 rat cortical neuron/glial cell culture stained with anti-vimentin (cat. 2107-VIM, red, 1:2000) and anti-GFAP antibody (cat. 621-GFAP, green, 1:5000). The blue is DAPI staining nuclear DNA. Vimentin is expressed alone in fibroblastic and developing cells and appear red. The astrocytes that appear red express only GFAP, while the golden yellow and yellow astrocytes express GFAP and Vimentin.
Mixed neuron/glial cultures stained with anti-vimentin (green, 1:500) and rabbit anti-GFAP antibody (cat. 620-GFAP, red, 1:1000). The blue stains nuclear DNA. Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).
Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.