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Antibodies

Anti-Microtubule Associated Protein 2 (MAP2) Antibody

Product Sizes
25 ul
£279.00
1100-MAP2-25UL
100 ul
£484.00
1100-MAP2-100UL
About this Product
SKU:
1100-MAP2
Additional Names:
DKFZp686I2148 antibody, MAP 2 antibody, MAP dendrite specific antibody, MAP-2 antibody, MAP2 antibody, MAP2A antibody, MAP2B antibody, MAP2C antibody, Microtubule associated protein 2 antibody, Microtubule-associated protein 2 antibody, MTAP2_HUMAN antibody
Application:
ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
19 mg/ml
Extra Details:
Our Anti-Microtubule Associated Protein 2 (MAP2) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Microtubule Associated Protein 2 (MAP2) and is total IgY fraction. It is great for use in WB, IHC, ICC., Phosphosolutions
Format:
Total IgY fraction
Formulation:
PBS + 10 mM NaN3.
Gene Details:
MAP2
Host:
Chicken/Avian
Immunogen:
Recombinant bovine MAP2 protein expressed in and purified from E. Coli.
Isotype:
IgY
Molecular Weight:
280
Physical State:
Liquid
Reactivities:
Bovine, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Specific for endogenous levels of the ~280 kDa MAP2 protein.
Storage Conditions:
-20[o]C Aliquot. Avoid freeze/thaw cycles. Store undiluted., 2-8[o]C Aliquot. Avoid freeze/thaw cycles. Store undiluted.
Type:
Antibody: Polyclonal Antibody
Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-MAP2 antibody (cat. 1100-MAP2, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2(cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
(A-B). Primary hippocampal neurons were stained with MAP2 (cat. 1100-MAP2, 1:1000) as a negative control under basal conditions (CTRL) and with picrotoxin treatment (PTX). Image from publication CC-BY-4.0. PMID: 37654026
Day 14 (D14) RGC-iNs stained with a DAPI, TAU, and MAP2 (cat. 1100-MAP2, 1:1000). Image from publication CC-BY-4.0. PMID: 37773257
Immunolabeling of mouse hippocampal cultures labeled with anti-MAP2 (cat. 1100-MAP2, 1:1000, red) and anti-synapsin (cat. 1925-SYNP , 1:1000, green).