Anti Extracellular Death Factor mouse monoclonal antibody
Product Sizes
0.1 ml
£ POA
MBS157892-0.1ML
About this Product
- SKU:
- MBS157892
- Additional Names:
- glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate 1-dehydrogenase; Description: mazEF is a toxin-antitoxin stress-induced module which is abundant on the chromosome of most bacteria including pathogens and most extensively studied in Escherichia coli. E Coli mazEF mediated cell death is a population phenomenon requiring the quorum-sensing (QS) -Extracellular Death Factor' (EDF) ; the E Coli peptide NNWNN. Penta-peptide NNWNN (EcEDF) enhances the endoribonucleolytic activity of E Coli toxin MazF. The newly discovered extracellular death factor (EDF) is a pentapeptide with the sequence NNWNN in Escherichia coli. It was reported that it participated in the cell death process mediated by toxin-antitoxin system mazEF. Reactive oxygen species (ROS) are recently considered as common factors for bactericidal antibiotics-mediated cell death. Previous study indicated that EDF could scavenge hydroxyl radicals and might act as a signal molecule with dual effects; oedeath and oesurvival . But the structure-activity relationship of EDF and the effects of EDF on the activity of antibiotics remain unclear. In the present study; our results indicated that tryptophan could be the key residue to the hydroxyl radicals-scavenging activity of EDF; and EDF could protect Escherichia coli from killing by bactericidal antibiotics; but not by DNA-damaging or bacteriostatic antibiotics. Our results could provide novel evidence to understand the role of EDF in drug-resistance.
- Application:
- ELISA, Immunohistochemistry
- translate.label.attr.clone:
- [93-D-4]
- Clonality:
- Monoclonal
- Concentration:
- 1 mg/ml
- Host:
- Mouse
- Isotype:
- IgG2b
- Physical State:
- Liquid. Supplied as solution form in PBS, pH7.4, containing 0.05% proclin300, 50% glycerol.
- Purity:
- Protein G purified from mice ascites
- Reactivities:
- Bacteria
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C/-70[o]C Avoid freeze/thaw cycles., 2-8[o]C Avoid freeze/thaw cycles.
- Supplier:
- MyBioSource
- Type:
- Antibody: Monoclonal Antibody
- Manufacturer's Data Sheet:MBS157892
