Cas9 Nickase D10A mRNA (5moU)
Product Sizes
500 μg
£ POA
HY-174499-500UG
About this Product
- SKU:
- HY-174499
- Extra Details:
- Cas9 Nickase D10A mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localization signal followed by a HA tag.Cas9 functions as part of the CRISPR (clustered regularly interspaced short palindromic repeats) genome editing system. In the CRISPR system; an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform the DNA cleavage. While wild-type Cas9 creates a double-stranded break at the target site; Cas9 nickase creates a single-stranded break. This favors homology-directed repair and decreases the occurrence of non-homologous end joining.
- Research Area:
- Others
- Shipping Conditions:
- Ambient
- Type:
- Molecular Biology Reagents: Nucleic Acids
