5-Bromo-3-indolyl β-D-galactopyranoside
Product Sizes
5 mg
£ POA
HY-137276-5MG
10 mg
£ POA
HY-137276-10MG
100 mg
£ POA
HY-137276-100MG
250 mg
£ POA
HY-137276-250MG
50 mg
£ POA
HY-137276-50MG
10mM/1mL
£ POA
HY-137276-10MM
500 mg
£ POA
HY-137276-500MG
About this Product
- SKU:
- HY-137276
- Additional Names:
- Bluo-Gal
- CAS Number:
- 97753-82-7
- Extra Details:
- 5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for β-galactosidase. 5-Bromo-3-indolyl β-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate; which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl β-D-galactopyranoside can specifically recognize bacterial β-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4; making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl β-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms; such as the localization analysis of β-galactosidase activity in mouse embryos or muscle tissues[1][2].
- Molecular Weight:
- 374.18
- Purity:
- 99.73
- References:
- [1]Gioglio L, et al. An improved method for beta-galactosidase activity detection on muscle tissue. A light and electron microscopic study. Ann Anat. 2002;184(2):153-157.|[2]Blanco MJ, et al. Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos. J Vis Exp. 2018 Jun 26;(136):57785.
- Research Area:
- Others
- Shipping Conditions:
- Ambient
- Storage Conditions:
- 4[o]C (Powder; protect from light)
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Fluorophores
- Pathway:
- Metabolic Enzyme/Protease;Others
