Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.000
View basket & checkout
Assay Kits & ELISAs

UbiTest Magnetic TUBE Elution Kit

Product Sizes
UbiTest Magnetic TUBE Elution Kit
£1,770.00
UM-0411M-1000
Academic Pricing
Please contact us for academic pricing.
Contact Us
About this Product
SKU:
UM-0411M-1000
Extra Details:
The UbiTest kit determines whether or not a target protein is poly-ubiquitinated. This powerful platform is an improvement on the most common method for investigating protein ubiquitination: immunoprecipitation and Western blot analysis. However, the substrate antibody often interacts differently with the poly-ubiquitinated forms of the substrate in the immuno-blotting step. This is due to epitope masking, reduced affinity, or changes in selectivity. A more definitive method for demonstrating protein ubiquitination is to couple immunoprecipitation with digestion by a broad spectrum deubiquitinase (DUB) prior to immunoblot analysis. An increased signal for the protein of interest (POI) after DUB treatment is a clear indication that the protein was ubiquitinated even if there was no clear reactivity in the untreated sample. To avoid potential problems arising from changes in immunoreactivity of the POI, the UbiTest assay utilizes TUBEs to pull-down the poly-ubiquitinated proteins. TUBEs (Tandem Ubiquitin Binding Entities) are engineered tandem ubiquitin-binding domains with dissociation constants for tetra-ubiquitin in the nanomolar range. TUBE1 has been demonstrated to bind to all 8 linkage types. \nKit Components \nTube 1 -- Magnetic TUBE1 - 1 mL of slurry, 100 uL slurry is recommended for 1-2 mg of total cell lysate pull down. \nTube 2 -- Wash buffer - 2 mL \nTube 3 -- Elution buffer - 1 mL \nTube 4 -- 10X Neutralization buffer (Add B Beta-mercaptoethanol to a final concentration of 10 mM before use, e.g. add 5 ul of 1 M B Beta-mercaptoethanol stock solution) - 0.5 mL \nTube 5 - Broad Spectrum DUB - 25 ug, 10 uM
Shipping Conditions:
Dry Ice
Storage Conditions:
2-8[o]C
Size:
10 pull-downs
Supplier:
LifeSensors
Type:
Assay Kits: Detection